{"id":"77e43f18-4306-590c-ac40-d355a1994c32","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-cyp1b1-scutellarein","predicate":"reported_relationship","statement":"Recombinant human CYP1B1 variants 1 and 3 hydroxylated apigenin at carbon 6 to scutellarein, with reported S50 values of 16 and 51 micromolar.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"0bcb9705-4a41-5c51-82b2-5cdd85ed01d5","mechanism_event_label":"Recombinant human CYP1B1 variants 1 and 3 hydroxylated apigenin at carbon 6 to scutellarein, with reported S50 values of 16 and 51 micromolar.","subject":{"id":"99d4e04f-4244-5dbc-9998-666db2ad4c74","slug":"cyp1b1","display_name":"Human cytochrome P450 1B1 / CYP1B1","entity_type_key":"protein"},"object":{"id":"89a73524-6293-56a6-8caf-6ecef2bc7c29","slug":"scutellarein","display_name":"Scutellarein / 6-hydroxyapigenin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0bcb9705-4a41-5c51-82b2-5cdd85ed01d5","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-cyp1b1-scutellarein-event","event_type":"biochemical_relationship","label":"Recombinant human CYP1B1 variants 1 and 3 hydroxylated apigenin at carbon 6 to scutellarein, with reported S50 values of 16 and 51 micromolar.","description":"### api-cyp1b1-scutellarein\n\nRecombinant human CYP1B1 variants 1 and 3 hydroxylated apigenin at carbon 6 to scutellarein, with reported S50 values of 16 and 51 micromolar.\nS50 is not automatically a Michaelis constant. Rates and variant identity are assay-specific.\nModel and exposure: Recombinant human enzymes; cooperative substrate-response curves.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Oxidation of Naringenin, Apigenin, and Genistein by Human Family 1 Cytochrome P450 Enzymes and Comparison of Interaction of Apigenin with Human P450 1B1.1 and Scutellaria P450 82D.1. | 2023 | https://pubmed.ncbi.nlm.nih.gov/37783573/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11497155/ | https://doi.org/10.1021/acs.chemrestox.3c00229 | Access: Indexed primary abstract and publisher-indexed Results; recombinant-enzyme selectivity, not complete human metabolic mass balance.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"99d4e04f-4244-5dbc-9998-666db2ad4c74","slug":"cyp1b1","display_name":"Human cytochrome P450 1B1 / CYP1B1","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"variants 1 and 3","sequence_order":1,"notes":""},{"entity":{"id":"89a73524-6293-56a6-8caf-6ecef2bc7c29","slug":"scutellarein","display_name":"Scutellarein / 6-hydroxyapigenin","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract and publisher-indexed Results; recombinant-enzyme selectivity, not complete human metabolic mass balance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human enzymes; cooperative substrate-response curves.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Recombinant human CYP1B1 variants 1 and 3 hydroxylated apigenin at carbon 6 to scutellarein, with reported S50 values of 16 and 51 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Oxidation of Naringenin, Apigenin, and Genistein by Human Family 1 Cytochrome P450 Enzymes and Comparison of Interaction of Apigenin with Human P450 1B1.1 and Scutellaria P450 82D.1. | https://pubmed.ncbi.nlm.nih.gov/37783573/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11497155/ | https://doi.org/10.1021/acs.chemrestox.3c00229","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-cyp1b1-scutellarein; S50 is not automatically a Michaelis constant. Rates and variant identity are assay-specific.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3b2faf15-7af7-5430-a413-a0aeb529830d","evidence_kind":"source_excerpt","locator":"Lines 98-104","start_line":98,"end_line":104,"excerpt":"### api-cyp1b1-scutellarein\n\nRecombinant human CYP1B1 variants 1 and 3 hydroxylated apigenin at carbon 6 to scutellarein, with reported S50 values of 16 and 51 micromolar.\nS50 is not automatically a Michaelis constant. Rates and variant identity are assay-specific.\nModel and exposure: Recombinant human enzymes; cooperative substrate-response curves.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Oxidation of Naringenin, Apigenin, and Genistein by Human Family 1 Cytochrome P450 Enzymes and Comparison of Interaction of Apigenin with Human P450 1B1.1 and Scutellaria P450 82D.1. | 2023 | https://pubmed.ncbi.nlm.nih.gov/37783573/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11497155/ | https://doi.org/10.1021/acs.chemrestox.3c00229 | Access: Indexed primary abstract and publisher-indexed Results; recombinant-enzyme selectivity, not complete human metabolic mass balance.","model_system":"Recombinant human enzymes; cooperative substrate-response curves.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}