{"id":"77dd9f0f-6f63-5e4e-b379-ac43f735edb4","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-bbua-lyase","predicate":"cleaves","statement":"BbuA converted gamma-butyrobetainyl-CoA to TMA and crotonyl-CoA in the reconstituted anaerobic pathway.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cc8b7ed8-4ae6-53ed-8360-618c6bd96d15","mechanism_event_label":"A microbial enzyme releases the trimethylamine group.","subject":{"id":"a3af3d49-078b-538c-a84f-a96ffb8cfb88","slug":"e-timonensis-bbua","display_name":"Emergencia timonensis BbuA / GbuA TMA-generating enzyme","entity_type_key":"protein"},"object":{"id":"c24c83c0-4378-53b7-85c5-fc4be448c79f","slug":"gamma-butyrobetainyl-coa","display_name":"Gamma-butyrobetainyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"cc8b7ed8-4ae6-53ed-8360-618c6bd96d15","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-bbua-lyase-event","event_type":"observed_relationship","label":"A microbial enzyme releases the trimethylamine group.","description":"BbuA converted gamma-butyrobetainyl-CoA to TMA and crotonyl-CoA in the reconstituted anaerobic pathway.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a3af3d49-078b-538c-a84f-a96ffb8cfb88","slug":"e-timonensis-bbua","display_name":"Emergencia timonensis BbuA / GbuA TMA-generating enzyme","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c24c83c0-4378-53b7-85c5-fc4be448c79f","slug":"gamma-butyrobetainyl-coa","display_name":"Gamma-butyrobetainyl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ffdcd3fa-0e47-5c2b-96e2-00b7d1d5674e","slug":"trimethylamine","display_name":"Trimethylamine / TMA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c8ce07a8-dfe0-5fb9-9529-f4fe2fddc134","slug":"crotonyl-coa","display_name":"Crotonyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text and pathway reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"E. timonensis BbuA biochemical characterization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Flavin participation is supported; detailed catalytic chemistry remained proposed in this paper.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A microbial enzyme releases the trimethylamine group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Elucidation of an anaerobic pathway for metabolism of l-carnitine-derived γ-butyrobetaine to trimethylamine in human gut bacteria. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34362844/ · DOI 10.1073/pnas.2101498118","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bd8e1d4f-c4c3-5dc9-8a2b-47ed6d905ae5","evidence_kind":"source_excerpt","locator":"Lines 378-384","start_line":378,"end_line":384,"excerpt":"## l-carnitine-bbua-lyase\nA microbial enzyme releases the trimethylamine group.\nBbuA converted gamma-butyrobetainyl-CoA to TMA and crotonyl-CoA in the reconstituted anaerobic pathway.\nModel: E. timonensis BbuA biochemical characterization.\nLimitations: Flavin participation is supported; detailed catalytic chemistry remained proposed in this paper.\nEvidence access: Primary full text and pathway reconstitution\nElucidation of an anaerobic pathway for metabolism of l-carnitine-derived γ-butyrobetaine to trimethylamine in human gut bacteria. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34362844/ · DOI 10.1073/pnas.2101498118","model_system":"E. timonensis BbuA biochemical characterization.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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