{"id":"77a6bb43-84b7-53b6-b1a5-28a47373889f","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-acyclo-rar","predicate":"weakly_activated","statement":"Acyclo-retinoic acid required 50 micromolar for RAR-beta2 promoter activation, a level the authors considered unphysiological.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"daa78b86-9890-5651-adae-ab541a670caa","mechanism_event_label":"A similarly named metabolite is not equivalent to vitamin A retinoic acid.","subject":{"id":"15643686-dd81-51bb-a9a7-60fd575894ca","slug":"acyclo-retinoic-acid","display_name":"Acyclo-retinoic acid","entity_type_key":"small_molecule"},"object":{"id":"753e100f-4061-5c77-9336-7f373195fffa","slug":"rarb-promoter-transactivation","display_name":"RAR-beta promoter transactivation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"daa78b86-9890-5651-adae-ab541a670caa","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-acyclo-rar-event","event_type":"biochemical_relationship","label":"A similarly named metabolite is not equivalent to vitamin A retinoic acid.","description":"Acyclo-retinoic acid required 50 micromolar for RAR-beta2 promoter activation, a level the authors considered unphysiological.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3a361e92-c843-5beb-bb20-21664c48ecc5","slug":"gja1","display_name":"Human connexin 43 / GJA1","entity_type_key":"protein"},"role":"communication_related_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"15643686-dd81-51bb-a9a7-60fd575894ca","slug":"acyclo-retinoic-acid","display_name":"Acyclo-retinoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"753e100f-4061-5c77-9336-7f373195fffa","slug":"rarb-promoter-transactivation","display_name":"RAR-beta promoter transactivation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/10620348.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dfd11c7c19dcd07e092a601ff44e9c900b5ecd717d3661ac2aca48d5cac72011\", \"start_char\": 0, \"end_char\": 1295, \"text_sha256\": \"dfd11c7c19dcd07e092a601ff44e9c900b5ecd717d3661ac2aca48d5cac72011\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Gap-junction and promoter/mRNA assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lycopene 0.1 micromolar; acyclo-retinoic acid 1 or 50 micromolar depending on endpoint","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Communication, mRNA stability and RAR reporter responses are distinct; very high metabolite doses are not physiological proof.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human fetal skin fibroblasts and reporter systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A similarly named metabolite is not equivalent to vitamin A retinoic acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p10620348] Stimulation of gap junctional communication: comparison of acyclo-retinoic acid and lycopene. (2000). https://pubmed.ncbi.nlm.nih.gov/10620348/ DOI: 10.1006/abbi.1999.1510","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Connexin-related intercellular communication","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"aa6c12bd-631a-5489-9caf-b29fa76e1217","evidence_kind":"source_excerpt","locator":"Lines 637-648","start_line":637,"end_line":648,"excerpt":"### lycopene-acyclo-rar\nAcyclo-retinoic acid required 50 micromolar for RAR-beta2 promoter activation, a level the authors considered unphysiological.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A similarly named metabolite is not equivalent to vitamin A retinoic acid.\norganism: Human fetal skin fibroblasts and reporter systems\ntissue_or_cell_type: Connexin-related intercellular communication\nexperimental_model: Gap-junction and promoter/mRNA assays\nlimitations: Communication, mRNA stability and RAR reporter responses are distinct; very high metabolite doses are not physiological proof.\nexposure: Lycopene 0.1 micromolar; acyclo-retinoic acid 1 or 50 micromolar depending on endpoint\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/10620348.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dfd11c7c19dcd07e092a601ff44e9c900b5ecd717d3661ac2aca48d5cac72011\", \"start_char\": 0, \"end_char\": 1295, \"text_sha256\": \"dfd11c7c19dcd07e092a601ff44e9c900b5ecd717d3661ac2aca48d5cac72011\"}\n[lycopene-p10620348] Stimulation of gap junctional communication: comparison of acyclo-retinoic acid and lycopene. (2000). https://pubmed.ncbi.nlm.nih.gov/10620348/ DOI: 10.1006/abbi.1999.1510","model_system":"Gap-junction and promoter/mRNA assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lycopene-p10620348] Stimulation of gap junctional communication: comparison of acyclo-retinoic acid and lycopene. (2000). https://pubmed.ncbi.nlm.nih.gov/10620348/ DOI: 10.1006/abbi.1999.1510","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d1e09874-8bde-548f-9664-26ad5eedb3c7","stable_key":"import-aad5a443-96e9-52eb-963a-812e90f9f896","title":"Lycopene: absorption, metabolism, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"9a53dd877de8b71d446f741026477c0fd70a38edac89f6f25339a371a627a59a","revision_id":"2e68d88d-a689-5959-9f9c-1552dd089c22","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}