{"id":"778503b1-4b3f-53ad-a04c-fb6abd431503","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-nfkb-regulation","predicate":"attenuates","statement":"Orthosilicic acid attenuated TNF-induced NF-kappaB activation in the osteoblast experimental program.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d2382f6a-4428-58bb-a9e0-b128e00f1fd0","mechanism_event_label":"A regulatory-RNA response was linked to lower inflammatory signaling.","subject":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"object":{"id":"64c0157b-7f2a-5ed7-8908-205f62be0753","slug":"osteoblast-nfkb-2016-silicate-assays","display_name":"NF-kappaB activation in the 2016 silicate osteoblast study","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d2382f6a-4428-58bb-a9e0-b128e00f1fd0","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-nfkb-regulation-event","event_type":"observed_relationship","label":"A regulatory-RNA response was linked to lower inflammatory signaling.","description":"Orthosilicic acid attenuated TNF-induced NF-kappaB activation in the osteoblast experimental program.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"64c0157b-7f2a-5ed7-8908-205f62be0753","slug":"osteoblast-nfkb-2016-silicate-assays","display_name":"NF-kappaB activation in the 2016 silicate osteoblast study","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8d309e8c-00a5-5788-a323-634f6d4de8d0","slug":"human-mir146a","display_name":"Human miR-146a","entity_type_key":"rna"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human MSC/mouse preosteoblast study; exact species for each downstream assay unresolved in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not assign all downstream experiments to human cells or infer an oral anti-inflammatory treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A regulatory-RNA response was linked to lower inflammatory signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orthosilicic acid, Si(OH)4, stimulates osteoblast differentiation in vitro by upregulating miR-146a to antagonize NF-κB activation. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27163405/ · DOI 10.1016/j.actbio.2016.05.007","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4576501e-d1b0-5afe-ae49-6d7ba454c636","evidence_kind":"source_excerpt","locator":"Lines 520-526","start_line":520,"end_line":526,"excerpt":"## silica-nfkb-regulation\nA regulatory-RNA response was linked to lower inflammatory signaling.\nOrthosilicic acid attenuated TNF-induced NF-kappaB activation in the osteoblast experimental program.\nModel: Human MSC/mouse preosteoblast study; exact species for each downstream assay unresolved in accessed abstract.\nLimitations: Do not assign all downstream experiments to human cells or infer an oral anti-inflammatory treatment.\nEvidence access: Primary abstract\nOrthosilicic acid, Si(OH)4, stimulates osteoblast differentiation in vitro by upregulating miR-146a to antagonize NF-κB activation. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27163405/ · DOI 10.1016/j.actbio.2016.05.007","model_system":"Human MSC/mouse preosteoblast study; exact species for each downstream assay unresolved in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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