{"id":"7733dd43-0362-54cc-80b0-2ce3e70a23c8","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:atic-faicar-cyclization","predicate":"cyclizes","statement":"Human ATIC has a separable IMP cyclohydrolase domain that converts FAICAR to IMP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0732195c-5497-5591-ac96-f52017fc6579","mechanism_event_label":"After the folate donation, ATIC closes the purine ring.","subject":{"id":"9dec8ed2-6916-5967-8a8c-243dd6d4d5f1","slug":"atic","display_name":"Human bifunctional ATIC","entity_type_key":"protein"},"object":{"id":"469c873f-d969-5bda-afab-a2129a0d32f4","slug":"faicar","display_name":"5-Formamidoimidazole-4-carboxamide ribonucleotide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0732195c-5497-5591-ac96-f52017fc6579","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:atic-faicar-cyclization-event","event_type":"biochemical_relationship","label":"After the folate donation, ATIC closes the purine ring.","description":"Human ATIC has a separable IMP cyclohydrolase domain that converts FAICAR to IMP.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"be10a2ad-cb5e-5562-896c-86d4f6f504b5","slug":"imp","display_name":"Inosine monophosphate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9dec8ed2-6916-5967-8a8c-243dd6d4d5f1","slug":"atic","display_name":"Human bifunctional ATIC","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"469c873f-d969-5bda-afab-a2129a0d32f4","slug":"faicar","display_name":"5-Formamidoimidazole-4-carboxamide ribonucleotide","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Truncation-domain activity mapping","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This second step does not consume another folate molecule.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"After the folate donation, ATIC closes the purine ring.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[rayl-1996] The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping (1996). https://pubmed.ncbi.nlm.nih.gov/8567683/ DOI: 10.1074/jbc.271.4.2225","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"aee0ecc2-f7f3-577b-add3-c0d115d28bec","evidence_kind":"source_excerpt","locator":"Lines 1022-1032","start_line":1022,"end_line":1032,"excerpt":"### atic-faicar-cyclization\nHuman ATIC has a separable IMP cyclohydrolase domain that converts FAICAR to IMP.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: After the folate donation, ATIC closes the purine ring.\norganism: Homo sapiens\ntissue_or_cell_type: Cell-free\nexperimental_model: Truncation-domain activity mapping\nlimitations: This second step does not consume another folate molecule.\nexposure: Assay conditions described in the linked primary study.\n[rayl-1996] The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping (1996). https://pubmed.ncbi.nlm.nih.gov/8567683/ DOI: 10.1074/jbc.271.4.2225","model_system":"Truncation-domain activity mapping","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [rayl-1996] The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping (1996). https://pubmed.ncbi.nlm.nih.gov/8567683/ DOI: 10.1074/jbc.271.4.2225","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}