{"id":"76d8072e-8608-5bbf-bebd-a27c12858232","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-tlr2-binding","predicate":"binds","statement":"Low-DM pectin bound TLR2 with results consistent with electrostatic interaction of free galacturonate groups and positively charged receptor regions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"179546b9-f97f-5555-8749-01b426483134","mechanism_event_label":"Exposed acidic groups can interact directly with an immune receptor.","subject":{"id":"d54fb89f-e04d-580a-aff2-a97bdab74c19","slug":"low-dm-lemon-pectin","display_name":"Low-methylesterified lemon pectin, DM7 preparation","entity_type_key":"chemical_species"},"object":{"id":"7d88fac7-eee3-541d-a202-e73c3f04186e","slug":"tlr2","display_name":"Human Toll-like receptor 2 / TLR2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"179546b9-f97f-5555-8749-01b426483134","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-tlr2-binding-event","event_type":"biochemical_relationship","label":"Exposed acidic groups can interact directly with an immune receptor.","description":"Low-DM pectin bound TLR2 with results consistent with electrostatic interaction of free galacturonate groups and positively charged receptor regions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"373900c7-b6e2-5577-9538-be1d528b9fd0","slug":"tlr1","display_name":"Human Toll-like receptor 1 / TLR1","entity_type_key":"protein"},"role":"receptor_partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"03136613-3585-5b09-b14d-95fab9d22bf2","slug":"tlr6","display_name":"Human Toll-like receptor 6 / TLR6","entity_type_key":"protein"},"role":"comparison_partner","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d54fb89f-e04d-580a-aff2-a97bdab74c19","slug":"low-dm-lemon-pectin","display_name":"Low-methylesterified lemon pectin, DM7 preparation","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7d88fac7-eee3-541d-a202-e73c3f04186e","slug":"tlr2","display_name":"Human Toll-like receptor 2 / TLR2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/pectin-research/29545800.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c194f301d46822febd69d3bc9768bd31de73e5dd2ca87f0d3b0e64cd4726dc65\", \"start_char\": 0, \"end_char\": 1540, \"text_sha256\": \"c194f301d46822febd69d3bc9768bd31de73e5dd2ca87f0d3b0e64cd4726dc65\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptor binding, reporter cells, human dendritic cells and mouse ileitis model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lemon pectin DM7 versus DM75; cell exposures 1-100 micrograms/mL; mice 3 mg/day before doxorubicin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Model-specific. 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(2018). https://pubmed.ncbi.nlm.nih.gov/29545800/ DOI: 10.3389/fimmu.2018.00383","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Innate immune receptors and intestine","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cffca25a-c611-59e8-beb5-fbff7f3970c3","evidence_kind":"source_excerpt","locator":"Lines 347-358","start_line":347,"end_line":358,"excerpt":"### pectin-tlr2-binding\nLow-DM pectin bound TLR2 with results consistent with electrostatic interaction of free galacturonate groups and positively charged receptor regions.\nCondition category: normal\nnutrient_topic: Pectin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Exposed acidic groups can interact directly with an immune receptor.\norganism: Human receptor/cell assays; Mus musculus separately scoped\ntissue_or_cell_type: Innate immune receptors and intestine\nexperimental_model: Receptor binding, reporter cells, human dendritic cells and mouse ileitis model\nlimitations: Model-specific. Binding and cytokine experiments do not establish prevention of human inflammatory disease. Lack of increased measured SCFAs does not exclude every microbial contribution.\nexposure: Lemon pectin DM7 versus DM75; cell exposures 1-100 micrograms/mL; mice 3 mg/day before doxorubicin\nevidence_span: {\"source_cache\": \"artifacts/pectin-research/29545800.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c194f301d46822febd69d3bc9768bd31de73e5dd2ca87f0d3b0e64cd4726dc65\", \"start_char\": 0, \"end_char\": 1540, \"text_sha256\": \"c194f301d46822febd69d3bc9768bd31de73e5dd2ca87f0d3b0e64cd4726dc65\"}\n[pectin-p29545800] Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2-1 and Prevents Doxorubicin-Induced Ileitis. 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