{"id":"76781500-f782-52d4-92c4-fdb620c1e116","stable_key":"4fef8d72-f107-5eb5-b2fb-483ca85465e0:k2-sxr-matn2","predicate":"regulates_transcription_of","statement":"MATN2 was identified as a primary SXR target responsive to vitamin K2 and rifampicin in osteoblastic cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a31436f8-81e2-528f-871e-ee9c43017e32","mechanism_event_label":"The receptor connects to a separately identifiable downstream gene.","subject":{"id":"f9deea3c-fcb8-51c8-9194-438c2ad6298b","slug":"nr1i2","display_name":"Human pregnane X receptor / SXR / PXR / NR1I2","entity_type_key":"protein"},"object":{"id":"ba0a6d9e-5285-5930-b09c-17e4327ce910","slug":"matn2","display_name":"Human matrilin-2 / MATN2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a31436f8-81e2-528f-871e-ee9c43017e32","stable_key":"4fef8d72-f107-5eb5-b2fb-483ca85465e0:k2-sxr-matn2-event","event_type":"biochemical_relationship","label":"The receptor connects to a separately identifiable downstream gene.","description":"MATN2 was identified as a primary SXR target responsive to vitamin K2 and rifampicin in osteoblastic cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b5cf80a2-c423-5ff5-806e-fa37ddaf7303","slug":"k2","display_name":"Vitamin K2 / menaquinone family","entity_type_key":"chemical_species"},"role":"tested_ligand","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f9deea3c-fcb8-51c8-9194-438c2ad6298b","slug":"nr1i2","display_name":"Human pregnane X receptor / SXR / PXR / NR1I2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ba0a6d9e-5285-5930-b09c-17e4327ce910","slug":"matn2","display_name":"Human matrilin-2 / MATN2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/k2-research/16606623.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a2c0e756d507fd0cef9dea48e3eedc193084378509ec54bce2c097f8491e4b45\", \"start_char\": 0, \"end_char\": 1266, \"text_sha256\": \"a2c0e756d507fd0cef9dea48e3eedc193084378509ec54bce2c097f8491e4b45\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Microarray, qPCR and gain/loss-of-function studies","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Vitamin K2 and rifampicin/SXR stimulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell transcription and collagen accumulation; not a human fracture study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin K2 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"k2","display_name":"Vitamin K2 / menaquinone family","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human osteoblastic MG63 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The receptor connects to a separately identifiable downstream gene.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[k2-p16606623] Steroid and xenobiotic receptor SXR mediates vitamin K2-activated transcription of extracellular matrix-related genes and collagen accumulation in osteoblastic cells. (2006). https://pubmed.ncbi.nlm.nih.gov/16606623/ DOI: 10.1074/jbc.m600896200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Extracellular-matrix gene regulation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"88884f6a-0e2b-5971-94e9-b228aa71f489","evidence_kind":"source_excerpt","locator":"Lines 734-745","start_line":734,"end_line":745,"excerpt":"### k2-sxr-matn2\nMATN2 was identified as a primary SXR target responsive to vitamin K2 and rifampicin in osteoblastic cells.\nCondition category: normal\nnutrient_topic: Vitamin K2 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The receptor connects to a separately identifiable downstream gene.\norganism: Human osteoblastic MG63 cells\ntissue_or_cell_type: Extracellular-matrix gene regulation\nexperimental_model: Microarray, qPCR and gain/loss-of-function studies\nlimitations: Cell transcription and collagen accumulation; not a human fracture study.\nexposure: Vitamin K2 and rifampicin/SXR stimulation\nevidence_span: {\"source_cache\": \"artifacts/k2-research/16606623.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a2c0e756d507fd0cef9dea48e3eedc193084378509ec54bce2c097f8491e4b45\", \"start_char\": 0, \"end_char\": 1266, \"text_sha256\": \"a2c0e756d507fd0cef9dea48e3eedc193084378509ec54bce2c097f8491e4b45\"}\n[k2-p16606623] Steroid and xenobiotic receptor SXR mediates vitamin K2-activated transcription of extracellular matrix-related genes and collagen accumulation in osteoblastic cells. (2006). https://pubmed.ncbi.nlm.nih.gov/16606623/ DOI: 10.1074/jbc.m600896200","model_system":"Microarray, qPCR and gain/loss-of-function studies","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [k2-p16606623] Steroid and xenobiotic receptor SXR mediates vitamin K2-activated transcription of extracellular matrix-related genes and collagen accumulation in osteoblastic cells. (2006). https://pubmed.ncbi.nlm.nih.gov/16606623/ DOI: 10.1074/jbc.m600896200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dfeae572-af3d-5042-a10e-e67596f9ab28","stable_key":"import-4fef8d72-f107-5eb5-b2fb-483ca85465e0","title":"Vitamin K2: menaquinone forms, carboxylation, recycling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"dc31aff21110d09b025c177e03b4c5ca8349d52bf5f962285fdbfcf660c0e4f6","revision_id":"850887db-34f6-55aa-9d3e-b8fd3a4a5de3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}