{"id":"75fc9734-c7dc-5f84-9924-46db34e781ed","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-enz-bvitamin-rat-hydrolysis","predicate":"deficiency_reduces","statement":"Serum from rats fed zinc-deficient diets showed reduced B-vitamin phosphate-ester hydrolysis activity in the study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"daf96a33-792f-50d8-ace8-7e95a0f81c79","mechanism_event_label":"Dietary zinc deficiency also reduced this measured enzyme activity in rat serum.","subject":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"object":{"id":"0eede301-252b-5c75-b19d-54cb0e8a201d","slug":"b-vitamin-phosphate-hydrolysis","display_name":"Extracellular B-vitamin phosphate-ester hydrolysis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"daf96a33-792f-50d8-ace8-7e95a0f81c79","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-enz-bvitamin-rat-hydrolysis-event","event_type":"biochemical_relationship","label":"Dietary zinc deficiency also reduced this measured enzyme activity in rat serum.","description":"Serum from rats fed zinc-deficient diets showed reduced B-vitamin phosphate-ester hydrolysis activity in the study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"b1_study_context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"b2_study_context","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0eede301-252b-5c75-b19d-54cb0e8a201d","slug":"b-vitamin-phosphate-hydrolysis","display_name":"Extracellular B-vitamin phosphate-ester hydrolysis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Membrane lysates of transiently transfected or endogenously expressing cells; serum of rats fed zinc-deficient diets","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Rats fed zinc-deficient diets; dietary content and duration not available in the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract-only extraction. Individual enzyme-reaction assignments, depletion concentrations/durations, quantitative effects and human clinical implications are not established here. Hydrolysis of extracellular vitamin phosphates is distinct from intracellular cofactor activation. The abstract does not identify which vitamin-specific endpoints were measurable in rat serum. The full-text search extract specifically said rat-serum B6 hydrolysis could not be measured; no rat B6 reaction claim or PLP participant is imported.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Dietary zinc deficiency also reduced this measured enzyme activity in rat serum.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-enz-bvitamins2024] Rate of hydrolysis of the phosphate esters of B vitamins is reduced by zinc deficiency: In vitro and in vivo. (2024). https://pubmed.ncbi.nlm.nih.gov/39279493/ DOI: 10.1096/fj.202401207r","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Rat serum assayed ex vivo","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"82944e2c-bc26-599f-af71-bcb31a7a41a6","evidence_kind":"source_excerpt","locator":"Lines 846-857","start_line":846,"end_line":857,"excerpt":"### zinc-enz-bvitamin-rat-hydrolysis\nSerum from rats fed zinc-deficient diets showed reduced B-vitamin phosphate-ester hydrolysis activity in the study.\nCondition category: nutrient_deficiency\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Dietary zinc deficiency also reduced this measured enzyme activity in rat serum.\norganism: Rattus norvegicus\ntissue_or_cell_type: Rat serum assayed ex vivo\nexperimental_model: Membrane lysates of transiently transfected or endogenously expressing cells; serum of rats fed zinc-deficient diets\nlimitations: Abstract-only extraction. Individual enzyme-reaction assignments, depletion concentrations/durations, quantitative effects and human clinical implications are not established here. Hydrolysis of extracellular vitamin phosphates is distinct from intracellular cofactor activation. The abstract does not identify which vitamin-specific endpoints were measurable in rat serum. The full-text search extract specifically said rat-serum B6 hydrolysis could not be measured; no rat B6 reaction claim or PLP participant is imported.\nexposure: Rats fed zinc-deficient diets; dietary content and duration not available in the abstract.\ncross_nutrient: true\n[zinc-enz-bvitamins2024] Rate of hydrolysis of the phosphate esters of B vitamins is reduced by zinc deficiency: In vitro and in vivo. (2024). https://pubmed.ncbi.nlm.nih.gov/39279493/ DOI: 10.1096/fj.202401207r","model_system":"Membrane lysates of transiently transfected or endogenously expressing cells; serum of rats fed zinc-deficient diets","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-enz-bvitamins2024] Rate of hydrolysis of the phosphate esters of B vitamins is reduced by zinc deficiency: In vitro and in vivo. (2024). https://pubmed.ncbi.nlm.nih.gov/39279493/ DOI: 10.1096/fj.202401207r","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}