{"id":"7586a0d5-2611-5c98-bf65-f19e31683c4c","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-fad-heat-protection","predicate":"protects_activity_of","statement":"FAD supplied before a 46 C, five-minute incubation preserved more MTHFR activity than FAD added after heating.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f6a99d8a-4b91-5978-a015-ce0218ffebe2","mechanism_event_label":"Flavin protected the enzyme during heat stress.","subject":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"object":{"id":"9fc32b1a-0f08-544c-a361-1c728203b6ec","slug":"mthfr","display_name":"Methylenetetrahydrofolate reductase / MTHFR","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f6a99d8a-4b91-5978-a015-ce0218ffebe2","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-fad-heat-protection-event","event_type":"biochemical_relationship","label":"Flavin protected the enzyme during heat stress.","description":"FAD supplied before a 46 C, five-minute incubation preserved more MTHFR activity than FAD added after heating.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"FAD nutrient precursor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9fc32b1a-0f08-544c-a361-1c728203b6ec","slug":"mthfr","display_name":"Methylenetetrahydrofolate reductase / MTHFR","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"B2-derived FAD affects folate-enzyme stability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human MTHFR; kinetics and structures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"FAD before versus after heating","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Heat challenge is not physiological riboflavin deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Flavin protected the enzyme during heat stress.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[froese-2018] Structural basis for the regulation of human 5,10-methylenetetrahydrofolate reductase by phosphorylation and S-adenosylmethionine inhibition (2018). https://pubmed.ncbi.nlm.nih.gov/29891918/ DOI: 10.1038/s41467-018-04735-2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified protein","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"597be600-6b3f-5069-8969-0818e685db27","evidence_kind":"source_excerpt","locator":"Lines 447-458","start_line":447,"end_line":458,"excerpt":"### folate-methyl-fad-heat-protection\nFAD supplied before a 46 C, five-minute incubation preserved more MTHFR activity than FAD added after heating.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Flavin protected the enzyme during heat stress.\norganism: Homo sapiens\ntissue_or_cell_type: Purified protein\nexperimental_model: Recombinant human MTHFR; kinetics and structures.\nlimitations: Heat challenge is not physiological riboflavin deficiency.\nexposure: FAD before versus after heating\ncross_nutrient: B2-derived FAD affects folate-enzyme stability.\n[froese-2018] Structural basis for the regulation of human 5,10-methylenetetrahydrofolate reductase by phosphorylation and S-adenosylmethionine inhibition (2018). https://pubmed.ncbi.nlm.nih.gov/29891918/ DOI: 10.1038/s41467-018-04735-2","model_system":"Recombinant human MTHFR; kinetics and structures.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [froese-2018] Structural basis for the regulation of human 5,10-methylenetetrahydrofolate reductase by phosphorylation and S-adenosylmethionine inhibition (2018). https://pubmed.ncbi.nlm.nih.gov/29891918/ DOI: 10.1038/s41467-018-04735-2","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}