{"id":"75694a0a-11a5-5caf-9cc6-3afed5581305","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iod-clin-dbs-tg-reference","predicate":"has-study-reference-distribution","statement":"The third and 97th percentiles of the standardized dried-blood-spot Tg assay were 4 and 40 micrograms/L in the 700 iodine-sufficient, euthyroid, anti-Tg-negative children.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"edb15c94-c157-5cd0-a91f-2ed499ac7bcc","mechanism_event_label":"The study established a reference distribution for a particular pediatric assay.","subject":{"id":"d5056639-73e7-5a8b-bb2d-47843774b6b2","slug":"dried-blood-spot-thyroglobulin-concentration","display_name":"Dried-blood-spot thyroglobulin concentration","entity_type_key":"cellular_process"},"object":{"id":"ab42f761-f2c0-52e6-876b-12df0d1d03d7","slug":"thyroglobulin","display_name":"Human thyroglobulin","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"edb15c94-c157-5cd0-a91f-2ed499ac7bcc","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iod-clin-dbs-tg-reference-event","event_type":"observed_intervention","label":"The study established a reference distribution for a particular pediatric assay.","description":"The third and 97th percentiles of the standardized dried-blood-spot Tg assay were 4 and 40 micrograms/L in the 700 iodine-sufficient, euthyroid, anti-Tg-negative children.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0e0ac6d1-3336-506a-9f69-5838ca1500ed","slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"},"role":"nutrient status used to select reference children","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d5056639-73e7-5a8b-bb2d-47843774b6b2","slug":"dried-blood-spot-thyroglobulin-concentration","display_name":"Dried-blood-spot thyroglobulin concentration","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ab42f761-f2c0-52e6-876b-12df0d1d03d7","slug":"thyroglobulin","display_name":"Human thyroglobulin","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary indexed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Assay-standardization study in 700 iodine-sufficient euthyroid anti-Tg-antibody-negative children aged 5–14 and separate 10-month iodized-salt intervention","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"International reference sample and longitudinal measurements before, 5 months and 10 months after salt iodization in deficient children.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay-specific pediatric reference distribution; not a universal adult/pregnancy threshold. 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(2006). https://pubmed.ncbi.nlm.nih.gov/16968789/ DOI: 10.1210/jc.2006-1370","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Dried whole-blood thyroglobulin assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"3003b606-70d8-5bba-bf0d-927e5c4c7ff7","evidence_kind":"source_excerpt","locator":"Lines 1594-1606","start_line":1594,"end_line":1606,"excerpt":"### iod-clin-dbs-tg-reference\nThe third and 97th percentiles of the standardized dried-blood-spot Tg assay were 4 and 40 micrograms/L in the 700 iodine-sufficient, euthyroid, anti-Tg-negative children.\nCondition category: biomarker_context\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The study established a reference distribution for a particular pediatric assay.\norganism: Homo sapiens\ntissue_or_cell_type: Dried whole-blood thyroglobulin assay\nexperimental_model: Assay-standardization study in 700 iodine-sufficient euthyroid anti-Tg-antibody-negative children aged 5–14 and separate 10-month iodized-salt intervention\nlimitations: Assay-specific pediatric reference distribution; not a universal adult/pregnancy threshold. Anti-Tg-antibody-positive children were excluded from the reference sample; antibody interference and other thyroid diseases remain relevant.\nexposure: International reference sample and longitudinal measurements before, 5 months and 10 months after salt iodization in deficient children.\ncross_nutrient: false\nevidence_location: Primary indexed abstract.\n[iod-clin-tg2006] Assessment of iodine status using dried blood spot thyroglobulin: development of reference material and establishment of an international reference range in iodine-sufficient children. (2006). https://pubmed.ncbi.nlm.nih.gov/16968789/ DOI: 10.1210/jc.2006-1370","model_system":"Assay-standardization study in 700 iodine-sufficient euthyroid anti-Tg-antibody-negative children aged 5–14 and separate 10-month iodized-salt intervention","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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