{"id":"75655d00-4766-52f8-b73b-69cc7f76c1c8","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-top1-enzyme","predicate":"decreases_in_recorded_comparison","statement":"Apigenin reduced recombinant human topoisomerase-I DNA relaxation: over 30% inhibition at 10 micromolar and over 80% at 100 micromolar.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2d703299-9158-565c-a292-4a604b2e89d8","mechanism_event_label":"Apigenin reduced recombinant human topoisomerase-I DNA relaxation: over 30% inhibition at 10 micromolar and over 80% at 100 micromolar.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"3bf1c845-ddcc-5ae9-8a94-b592cdc76001","slug":"human-top1","display_name":"Human DNA topoisomerase I / TOP1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2d703299-9158-565c-a292-4a604b2e89d8","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-top1-enzyme-event","event_type":"biochemical_relationship","label":"Apigenin reduced recombinant human topoisomerase-I DNA relaxation: over 30% inhibition at 10 micromolar and over 80% at 100 micromolar.","description":"### api-top1-enzyme\n\nApigenin reduced recombinant human topoisomerase-I DNA relaxation: over 30% inhibition at 10 micromolar and over 80% at 100 micromolar.\nFunctional inhibition plus modeling does not supply an independently measured binding affinity.\nModel and exposure: Cell-free DNA-unwinding/relaxation assay.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin directly interacts with and inhibits topoisomerase 1 to upregulate CD26/DPP4 on colorectal carcinoma cells. | 2022 | https://pubmed.ncbi.nlm.nih.gov/36618939/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC9815539/ | https://doi.org/10.3389/fphar.2022.1086894 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin concentration series","sequence_order":0,"notes":""},{"entity":{"id":"3bf1c845-ddcc-5ae9-8a94-b592cdc76001","slug":"human-top1","display_name":"Human DNA topoisomerase I / TOP1","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin concentration series\", \"comparator\": \"Vehicle\", \"endpoint\": \"Apigenin reduced recombinant human topoisomerase-I DNA relaxation: over 30% inhibition at 10 micromolar and over 80% at 100 micromolar.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Cell-free DNA-unwinding/relaxation assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin reduced recombinant human topoisomerase-I DNA relaxation: over 30% inhibition at 10 micromolar and over 80% at 100 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Apigenin directly interacts with and inhibits topoisomerase 1 to upregulate CD26/DPP4 on colorectal carcinoma cells. | https://pubmed.ncbi.nlm.nih.gov/36618939/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC9815539/ | https://doi.org/10.3389/fphar.2022.1086894","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-top1-enzyme; Functional inhibition plus modeling does not supply an independently measured binding affinity.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5bacff84-30cb-53cf-aab0-6c3cec377d17","evidence_kind":"source_excerpt","locator":"Lines 708-714","start_line":708,"end_line":714,"excerpt":"### api-top1-enzyme\n\nApigenin reduced recombinant human topoisomerase-I DNA relaxation: over 30% inhibition at 10 micromolar and over 80% at 100 micromolar.\nFunctional inhibition plus modeling does not supply an independently measured binding affinity.\nModel and exposure: Cell-free DNA-unwinding/relaxation assay.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin directly interacts with and inhibits topoisomerase 1 to upregulate CD26/DPP4 on colorectal carcinoma cells. | 2022 | https://pubmed.ncbi.nlm.nih.gov/36618939/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC9815539/ | https://doi.org/10.3389/fphar.2022.1086894 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Cell-free DNA-unwinding/relaxation assay.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}