{"id":"74d0924b-eab5-5379-a4a7-d856cc4aa679","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-tubulin-glutamylation","predicate":"catalyzes","statement":"Recombinant mouse TTLL7 favored beta-tubulin polyglutamylation in vitro and when expressed in HEK293T cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"dd7a5d8c-e676-5c4b-a062-44f2091ee86f","mechanism_event_label":"Glutamate can be added in side chains that modify a structural protein.","subject":{"id":"6aadc85e-7728-58e5-a01f-99f0dc8c871f","slug":"mouse-ttll7","display_name":"Mouse tubulin polyglutamylase / Ttll7","entity_type_key":"protein"},"object":{"id":"d2b0a13b-989d-50a8-9b83-ce30a4e710be","slug":"mouse-ttll7-beta-tubulin-glutamylation","display_name":"Beta-tubulin polyglutamylation in mouse TTLL7 assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dd7a5d8c-e676-5c4b-a062-44f2091ee86f","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-tubulin-glutamylation-event","event_type":"observed_relationship","label":"Glutamate can be added in side chains that modify a structural protein.","description":"Recombinant mouse TTLL7 favored beta-tubulin polyglutamylation in vitro and when expressed in HEK293T cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6aadc85e-7728-58e5-a01f-99f0dc8c871f","slug":"mouse-ttll7","display_name":"Mouse tubulin polyglutamylase / Ttll7","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d2b0a13b-989d-50a8-9b83-ce30a4e710be","slug":"mouse-ttll7-beta-tubulin-glutamylation","display_name":"Beta-tubulin polyglutamylation in mouse TTLL7 assays","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and full-text construct methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse cDNA constructs, recombinant biochemistry and human host-cell expression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This protein modification differs from a free-glutamate receptor signal; the beta-tubulin isoform is not assigned beyond the study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Glutamate can be added in side chains that modify a structural protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16901895/ · DOI 10.1074/jbc.M603984200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a8c266e0-023a-582f-9973-517bd9fa27ae","evidence_kind":"source_excerpt","locator":"Lines 410-416","start_line":410,"end_line":416,"excerpt":"## glutamate-tubulin-glutamylation\nGlutamate can be added in side chains that modify a structural protein.\nRecombinant mouse TTLL7 favored beta-tubulin polyglutamylation in vitro and when expressed in HEK293T cells.\nModel: Mouse cDNA constructs, recombinant biochemistry and human host-cell expression.\nLimitations: This protein modification differs from a free-glutamate receptor signal; the beta-tubulin isoform is not assigned beyond the study.\nEvidence access: Primary abstract and full-text construct methods\nTTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16901895/ · DOI 10.1074/jbc.M603984200","model_system":"Mouse cDNA constructs, recombinant biochemistry and human host-cell expression.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3da83bd75fddeb96d17af72d7e2ae04344779957650f266521f6c8ebd59fb54f","revision_id":"55196c72-94e4-540a-a869-81d35647b04a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}