{"id":"74c60726-1fc4-5441-86ea-9001228788b2","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-rgi-rhamnose","predicate":"hydrolyzes","statement":"BT4145 is an RG-I-specific GH106 alpha-L-rhamnosidase acting by inversion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7a81a112-ca25-5a71-94d0-24a3094648b7","mechanism_event_label":"This enzyme removes rhamnose links in the RG-I sequence.","subject":{"id":"1969330b-ed73-5910-a499-6b8854f94c2c","slug":"bt-bt4145","display_name":"Bacteroides thetaiotaomicron BT4145, GH106 alpha-rhamnosidase","entity_type_key":"protein"},"object":{"id":"525e567a-bc99-5ba2-b015-8c34e1384d1d","slug":"rgi-oligosaccharides","display_name":"Rhamnogalacturonan-I-derived oligosaccharides","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"7a81a112-ca25-5a71-94d0-24a3094648b7","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-rgi-rhamnose-event","event_type":"biochemical_relationship","label":"This enzyme removes rhamnose links in the RG-I sequence.","description":"BT4145 is an RG-I-specific GH106 alpha-L-rhamnosidase acting by inversion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1969330b-ed73-5910-a499-6b8854f94c2c","slug":"bt-bt4145","display_name":"Bacteroides thetaiotaomicron BT4145, GH106 alpha-rhamnosidase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"525e567a-bc99-5ba2-b015-8c34e1384d1d","slug":"rgi-oligosaccharides","display_name":"Rhamnogalacturonan-I-derived oligosaccharides","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/pectin-research/29255254.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"514f1ffe2924a1c555e3ecea968fb7260d0f55aa1b22c057697dbc59676c5796\", \"start_char\": 13259, \"end_char\": 14426, \"text_sha256\": \"2e55a6b2c9c29b9f82f572f66f439f63e58c9619942be2ed1b35ee8e7a193938\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified enzymes, structural biology and targeted bacterial deletions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Defined pectic substrates; growth media and recombinant-enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Bacterial machinery, not human digestive enzymes. Gene dependence is substrate- and strain-specific; no clinical microbiome threshold follows.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pectin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pectin","display_name":"Pectin, structurally heterogeneous plant polysaccharides","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Bacteroides thetaiotaomicron","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This enzyme removes rhamnose links in the RG-I sequence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[pectin-p29255254] Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. (2018). https://pubmed.ncbi.nlm.nih.gov/29255254/ DOI: 10.1038/s41564-017-0079-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell surface and periplasm","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ace63911-92d7-549f-872b-2a62d7a6b081","evidence_kind":"source_excerpt","locator":"Lines 204-215","start_line":204,"end_line":215,"excerpt":"### pectin-rgi-rhamnose\nBT4145 is an RG-I-specific GH106 alpha-L-rhamnosidase acting by inversion.\nCondition category: normal\nnutrient_topic: Pectin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This enzyme removes rhamnose links in the RG-I sequence.\norganism: Bacteroides thetaiotaomicron\ntissue_or_cell_type: Cell surface and periplasm\nexperimental_model: Purified enzymes, structural biology and targeted bacterial deletions\nlimitations: Bacterial machinery, not human digestive enzymes. Gene dependence is substrate- and strain-specific; no clinical microbiome threshold follows.\nexposure: Defined pectic substrates; growth media and recombinant-enzyme assays\nevidence_span: {\"source_cache\": \"artifacts/pectin-research/29255254.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"514f1ffe2924a1c555e3ecea968fb7260d0f55aa1b22c057697dbc59676c5796\", \"start_char\": 13259, \"end_char\": 14426, \"text_sha256\": \"2e55a6b2c9c29b9f82f572f66f439f63e58c9619942be2ed1b35ee8e7a193938\"}\n[pectin-p29255254] Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. 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