{"id":"74bc5e31-e296-5958-b50d-62312077ec63","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-gstp-retention","predicate":"increased","statement":"GSTP1-1 expression alone accelerated sulforaphane/conjugate accumulation and increased ARE reporter induction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d5c0744a-a87b-55f9-ae76-c8bc6a5a93aa","mechanism_event_label":"Conjugation can initially retain the exposure inside cells.","subject":{"id":"3574cefe-a463-5a19-83f0-d8efc9b5c908","slug":"gstp1","display_name":"Human glutathione S-transferase P1 / GSTP1","entity_type_key":"protein"},"object":{"id":"5b74cc38-d5d1-50bd-8542-8d9b3c570df5","slug":"sulforaphane-intracellular-accumulation","display_name":"Intracellular sulforaphane plus glutathione-conjugate accumulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d5c0744a-a87b-55f9-ae76-c8bc6a5a93aa","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-gstp-retention-event","event_type":"biochemical_relationship","label":"Conjugation can initially retain the exposure inside cells.","description":"GSTP1-1 expression alone accelerated sulforaphane/conjugate accumulation and increased ARE reporter induction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"conjugating_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e91e547a-a84c-5d7d-afbd-89e38a950c16","slug":"sulforaphane-glutathione","display_name":"Sulforaphane-glutathione conjugate / SFN-GSH","entity_type_key":"small_molecule"},"role":"conjugate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3574cefe-a463-5a19-83f0-d8efc9b5c908","slug":"gstp1","display_name":"Human glutathione S-transferase P1 / GSTP1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5b74cc38-d5d1-50bd-8542-8d9b3c570df5","slug":"sulforaphane-intracellular-accumulation","display_name":"Intracellular sulforaphane plus glutathione-conjugate accumulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/18204073.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0fcd98974f9fe12b2ca7c4326f94eddf4f20de4ac2ae6fdebb64de0efb1d8be9\", \"start_char\": 0, \"end_char\": 1894, \"text_sha256\": \"0fcd98974f9fe12b2ca7c4326f94eddf4f20de4ac2ae6fdebb64de0efb1d8be9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transgenic transporter/enzyme comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane; GSH depletion control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered cancer cells; enzyme expression and efflux can have opposing effects without being a scientific contradiction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human MCF7 cells with GSTP1 and/or MRP1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Conjugation can initially retain the exposure inside cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p18204073] Expression of MRP1 and GSTP1-1 modulate the acute cellular response to treatment with the chemopreventive isothiocyanate, sulforaphane. (2008). https://pubmed.ncbi.nlm.nih.gov/18204073/ DOI: 10.1093/carcin/bgn013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intracellular retention, ARE induction and Nrf2 persistence","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f86b7250-7ff3-58aa-a6af-4f22ffa4b6ce","evidence_kind":"source_excerpt","locator":"Lines 1009-1020","start_line":1009,"end_line":1020,"excerpt":"### sulforaphane-gstp-retention\nGSTP1-1 expression alone accelerated sulforaphane/conjugate accumulation and increased ARE reporter induction.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Conjugation can initially retain the exposure inside cells.\norganism: Human MCF7 cells with GSTP1 and/or MRP1\ntissue_or_cell_type: Intracellular retention, ARE induction and Nrf2 persistence\nexperimental_model: Transgenic transporter/enzyme comparison\nlimitations: Engineered cancer cells; enzyme expression and efflux can have opposing effects without being a scientific contradiction.\nexposure: Sulforaphane; GSH depletion control\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/18204073.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0fcd98974f9fe12b2ca7c4326f94eddf4f20de4ac2ae6fdebb64de0efb1d8be9\", \"start_char\": 0, \"end_char\": 1894, \"text_sha256\": \"0fcd98974f9fe12b2ca7c4326f94eddf4f20de4ac2ae6fdebb64de0efb1d8be9\"}\n[sulforaphane-p18204073] Expression of MRP1 and GSTP1-1 modulate the acute cellular response to treatment with the chemopreventive isothiocyanate, sulforaphane. (2008). https://pubmed.ncbi.nlm.nih.gov/18204073/ DOI: 10.1093/carcin/bgn013","model_system":"Transgenic transporter/enzyme comparison","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sulforaphane-p18204073] Expression of MRP1 and GSTP1-1 modulate the acute cellular response to treatment with the chemopreventive isothiocyanate, sulforaphane. (2008). https://pubmed.ncbi.nlm.nih.gov/18204073/ DOI: 10.1093/carcin/bgn013","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"a4bafbf7-f117-53c9-a6d7-8fb25e4aca72","stable_key":"import-44eaeae5-557a-552b-8998-884f30462e2a","title":"Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f355c492a6c35673d8260b1328667fe206e5315b9fcaa5aeff53d80373c7ef97","revision_id":"f2c47d71-34cd-5160-8e92-fe196b576707","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}