{"id":"747e522e-3c80-5298-80ae-e810678c971e","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-gad-inhibitor-null","predicate":"did_not_prevent_theanine_reduction_of","statement":"The glutamate-decarboxylase inhibitor did not prevent theanine protection in the tested mouse model.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ca22b0ab-e9b7-513a-8d56-a0c4af4a8cee","mechanism_event_label":"Receptor dependence cannot be simplified to proof that newly synthesized GABA caused the effect.","subject":{"id":"7b319eb3-8548-5df2-a486-e207a8da9e9a","slug":"three-mercaptopropionic-acid","display_name":"3-Mercaptopropionic acid","entity_type_key":"small_molecule"},"object":{"id":"bafda041-64f5-503c-8249-f72f47c7e456","slug":"mouse-ischemic-infarct-size","display_name":"Mouse cerebral infarct size after focal ischemia","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ca22b0ab-e9b7-513a-8d56-a0c4af4a8cee","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-gad-inhibitor-null-event","event_type":"biochemical_relationship","label":"Receptor dependence cannot be simplified to proof that newly synthesized GABA caused the effect.","description":"The glutamate-decarboxylase inhibitor did not prevent theanine protection in the tested mouse model.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7b319eb3-8548-5df2-a486-e207a8da9e9a","slug":"three-mercaptopropionic-acid","display_name":"3-Mercaptopropionic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bafda041-64f5-503c-8249-f72f47c7e456","slug":"mouse-ischemic-infarct-size","display_name":"Mouse cerebral infarct size after focal ischemia","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/17928735.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5568750575b7baedd13ae86dfa48298b5332447016c7b979f6fedf865ce00db3\", \"start_char\": 0, \"end_char\": 696, \"text_sha256\": \"5568750575b7baedd13ae86dfa48298b5332447016c7b979f6fedf865ce00db3\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Focal cerebral ischemia and pharmacological pathway blockade","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Theanine 1 mg/kg; four-hour MCA occlusion; bicuculline or 3-mercaptopropionic acid","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preclinical acute brain injury, not human stroke treatment or proof theanine directly activates GABA-A receptors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Receptor dependence cannot be simplified to proof that newly synthesized GABA caused the effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p17928735] Involvement of GABA(A) receptors in the neuroprotective effect of theanine on focal cerebral ischemia in mice. 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