{"id":"743701c3-ac30-5cba-9c1c-25456bf3c8da","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:aldh1l1-serine-glycine","predicate":"changes","statement":"ALDH1L1-expressing HuH-7 cells had lower serine and higher glycine than controls.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a76d3a3d-defe-5585-87cd-aa4426bac584","mechanism_event_label":"Changing folate-carbon disposal reshaped amino-acid pools.","subject":{"id":"546aed58-bcd0-5ed1-8b43-c196155dcbac","slug":"aldh1l1","display_name":"Human ALDH1L1","entity_type_key":"protein"},"object":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a76d3a3d-defe-5585-87cd-aa4426bac584","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:aldh1l1-serine-glycine-event","event_type":"observed_intervention","label":"Changing folate-carbon disposal reshaped amino-acid pools.","description":"ALDH1L1-expressing HuH-7 cells had lower serine and higher glycine than controls.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"increased amino acid","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"546aed58-bcd0-5ed1-8b43-c196155dcbac","slug":"aldh1l1","display_name":"Human ALDH1L1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Cell metabolomics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Engineered ALDH1L1 expression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pool changes alone do not quantify compartment-specific SHMT flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Changing folate-carbon disposal reshaped amino-acid pools.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sasaki-2023] One-carbon metabolizing enzyme ALDH1L1 influences mitochondrial metabolism through 5-aminoimidazole-4-carboxamide ribonucleotide accumulation and serine depletion, contributing to tumor suppression (2023). https://pubmed.ncbi.nlm.nih.gov/37596270/ DOI: 10.1038/s41598-023-38142-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HuH-7 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e7ede151-3517-592f-9371-a17a6598844e","evidence_kind":"source_excerpt","locator":"Lines 1084-1094","start_line":1084,"end_line":1094,"excerpt":"### aldh1l1-serine-glycine\nALDH1L1-expressing HuH-7 cells had lower serine and higher glycine than controls.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Changing folate-carbon disposal reshaped amino-acid pools.\norganism: Homo sapiens\ntissue_or_cell_type: HuH-7 cells\nexperimental_model: Cell metabolomics\nlimitations: Pool changes alone do not quantify compartment-specific SHMT flux.\nexposure: Engineered ALDH1L1 expression.\n[sasaki-2023] One-carbon metabolizing enzyme ALDH1L1 influences mitochondrial metabolism through 5-aminoimidazole-4-carboxamide ribonucleotide accumulation and serine depletion, contributing to tumor suppression (2023). https://pubmed.ncbi.nlm.nih.gov/37596270/ DOI: 10.1038/s41598-023-38142-5","model_system":"Cell metabolomics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sasaki-2023] One-carbon metabolizing enzyme ALDH1L1 influences mitochondrial metabolism through 5-aminoimidazole-4-carboxamide ribonucleotide accumulation and serine depletion, contributing to tumor suppression (2023). https://pubmed.ncbi.nlm.nih.gov/37596270/ DOI: 10.1038/s41598-023-38142-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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