{"id":"74272494-15a1-52d0-ae48-69a3e1949a70","stable_key":"d39c4b36-9a88-5f52-8e6b-93a248dc4bfc:hericenones-erinacines-hericenone-cde-null","predicate":"does_not_induce","statement":"Purified hericenones C, D and E failed to promote NGF gene expression in the 1321N1 assay that responded to the whole extract.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"2a5025cf-594a-5bc7-815e-1090fac92698","mechanism_event_label":"Purified hericenones C, D and E failed to promote NGF gene expression in the 1321N1 assay that responded to the whole extract.","subject":{"id":"631eaf08-b37b-54d0-910c-7ab40cae5b96","slug":"hericenone-c","display_name":"Hericenone C","entity_type_key":"small_molecule"},"object":{"id":"73b34833-841f-5eb2-addb-08555421b9eb","slug":"human-astrocytoma-ngf-expression","display_name":"NGF expression and secretion in human 1321N1 astrocytoma cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2a5025cf-594a-5bc7-815e-1090fac92698","stable_key":"d39c4b36-9a88-5f52-8e6b-93a248dc4bfc:hericenones-erinacines-hericenone-cde-null-event","event_type":"observed_relationship","label":"Purified hericenones C, D and E failed to promote NGF gene expression in the 1321N1 assay that responded to the whole extract.","description":"Purified hericenones C, D and E failed to promote NGF gene expression in the 1321N1 assay that responded to the whole extract.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"631eaf08-b37b-54d0-910c-7ab40cae5b96","slug":"hericenone-c","display_name":"Hericenone C","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"73b34833-841f-5eb2-addb-08555421b9eb","slug":"human-astrocytoma-ngf-expression","display_name":"NGF expression and secretion in human 1321N1 astrocytoma cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"729609d4-cf24-5047-8fcb-81e44b7ad624","slug":"hericenone-d","display_name":"Hericenone D","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e6b83acb-bf46-5777-a085-02025da533a8","slug":"hericenone-e","display_name":"Hericenone E","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"167e6f4f-4111-5898-a3ad-18b695d2cda2","slug":"hericium-ethanol-extract-2008","display_name":"Hericium erinaceus ethanol extract used in the 2008 NGF study","entity_type_key":"botanical"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Purified hericenones C, D and E","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human 1321N1 astrocytoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This source-specific null prevents assigning the extract's NGF induction to those three molecules; other assays may differ.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hericenones & Erinacines chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hericenones-erinacines","display_name":"Hericenones and erinacines","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human 1321N1 astrocytoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Purified hericenones C, D and E failed to promote NGF gene expression in the 1321N1 assay that responded to the whole extract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Nerve growth factor-inducing activity of Hericium erinaceus in 1321N1 human astrocytoma cells. 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