{"id":"73e4ee94-276b-58d6-9557-75dc7179ca6b","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-parent-oat-null","predicate":"does_not_inhibit_in_assay","statement":"Stevioside did not inhibit human OAT3-mediated estrone-sulfate uptake or OAT1-mediated para-aminohippurate uptake, whereas steviol did.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"42ed4e81-8c9f-5f67-804d-3f2fbab152fa","mechanism_event_label":"The parent sweetener and its metabolite do not share every transport interaction.","subject":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"object":{"id":"4606352d-ad2e-580f-87eb-e0ccf0d02fd9","slug":"slc22a8","display_name":"Human organic anion transporter 3 / SLC22A8","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"42ed4e81-8c9f-5f67-804d-3f2fbab152fa","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-parent-oat-null-event","event_type":"observed_relationship","label":"The parent sweetener and its metabolite do not share every transport interaction.","description":"Stevioside did not inhibit human OAT3-mediated estrone-sulfate uptake or OAT1-mediated para-aminohippurate uptake, whereas steviol did.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4606352d-ad2e-580f-87eb-e0ccf0d02fd9","slug":"slc22a8","display_name":"Human organic anion transporter 3 / SLC22A8","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4872be13-147b-53b0-bd51-c717906aa430","slug":"slc22a6","display_name":"Human organic anion transporter 1 / SLC22A6","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human OAT expression in Xenopus oocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A negative uptake-inhibition assay does not rule out all other transporters.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The parent sweetener and its metabolite do not share every transport interaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8). · 2005 · https://pubmed.ncbi.nlm.nih.gov/15644426/ · DOI 10.1124/jpet.104.080366","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"65efbf55-e37b-553e-8adc-b370cf5d8afb","evidence_kind":"source_excerpt","locator":"Lines 114-120","start_line":114,"end_line":120,"excerpt":"## stevia-parent-oat-null\nThe parent sweetener and its metabolite do not share every transport interaction.\nStevioside did not inhibit human OAT3-mediated estrone-sulfate uptake or OAT1-mediated para-aminohippurate uptake, whereas steviol did.\nModel: Human OAT expression in Xenopus oocytes.\nLimitations: A negative uptake-inhibition assay does not rule out all other transporters.\nEvidence access: Primary abstract\nTransport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8). · 2005 · https://pubmed.ncbi.nlm.nih.gov/15644426/ · DOI 10.1124/jpet.104.080366","model_system":"Human OAT expression in Xenopus oocytes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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