{"id":"73df2ca3-eb27-5f32-8790-d6682cab102e","stable_key":"research:stat5-inhibits-tfh-differentiation","predicate":"inhibits","statement":"Sustained STAT5 activity inhibited BCL6-associated Tfh differentiation in the studied model.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"negative","is_public":true,"mechanism_event_id":"5d59d22d-ec03-56c2-9dc1-c43d17e8da65","mechanism_event_label":"More STAT5 signaling reduced the Tfh cell fate.","subject":{"id":"d8de1e6a-fdad-52a0-8b9d-c9ea510a0238","slug":"stat5","display_name":"STAT5","entity_type_key":"protein_family"},"object":{"id":"41da76dd-b500-58e8-bf3d-e2fa55fb8978","slug":"tfh-differentiation","display_name":"Follicular helper T-cell differentiation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5d59d22d-ec03-56c2-9dc1-c43d17e8da65","stable_key":"research:stat5-inhibits-tfh-differentiation","event_type":"signaling-perturbation","label":"More STAT5 signaling reduced the Tfh cell fate.","description":"Sustained STAT5 activity inhibited BCL6-associated Tfh differentiation in the studied model.","status":"active","compartment":null,"participants":[{"entity":{"id":"d8de1e6a-fdad-52a0-8b9d-c9ea510a0238","slug":"stat5","display_name":"STAT5","entity_type_key":"protein_family"},"role":"perturbed-factor","stoichiometry":null,"state_label":"constitutively active","sequence_order":0,"notes":""},{"entity":{"id":"41da76dd-b500-58e8-bf3d-e2fa55fb8978","slug":"tfh-differentiation","display_name":"Follicular helper T-cell differentiation","entity_type_key":"cellular_process"},"role":"measured-process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9c9d949e-0097-5f4f-8720-6da687299eac","slug":"bcl6","display_name":"BCL6","entity_type_key":"protein"},"role":"fate-marker","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"transferred antigen-specific CD4 T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"LCMV infection","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not all IL-2 effects are inhibitory.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ec52493a-d489-5e86-a25b-fa789b554b8f","evidence_kind":"curated_literature_summary","locator":"lines 930-940","start_line":930,"end_line":940,"excerpt":"## stat5-inhibits-tfh-differentiation\n\nMore STAT5 signaling reduced the Tfh cell fate.\n\nSustained STAT5 activity inhibited BCL6-associated Tfh differentiation in the studied model.\n\nOrganism: mouse\nCell type: transferred antigen-specific CD4 T cells\nExperimental model: LCMV infection\nLimitations: Not all IL-2 effects are inhibitory.\nPrimary reference: [STAT5 is a potent negative regulator of TFH cell differentiation](https://pmc.ncbi.nlm.nih.gov/articles/PMC3281266/)","model_system":"LCMV infection","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"t-cell-fate","plain_language":"More STAT5 signaling reduced the Tfh cell fate.","evidence_scope":"direct_experimental","papers":[{"key":"johnston-2012-stat5-tfh","title":"STAT5 is a potent negative regulator of TFH cell differentiation","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3281266/","doi":"10.1084/jem.20111174","year":2012,"model":"Mouse LCMV infection, transferred antigen-specific CD4 cells and genetic perturbations","summary":"Tests IL-2/STAT5 suppression of Tfh differentiation and its dependence on Blimp-1."}]}}