{"id":"73ddcea4-b26d-540a-b49f-5ef5e18bcddf","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-yrs-tlr2","predicate":"activates","statement":"Extracellular YARS1 activated TLR2 signaling and inflammatory mediator release in human monocyte/macrophage preparations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d0589835-e5e0-5002-80eb-a5d056fd57f9","mechanism_event_label":"A protein used for translation also signals outside cells.","subject":{"id":"1e70f8cb-144f-5c63-b862-0c8670840077","slug":"yars1","display_name":"Human cytosolic tyrosyl-tRNA synthetase / YARS1","entity_type_key":"protein"},"object":{"id":"7d88fac7-eee3-541d-a202-e73c3f04186e","slug":"tlr2","display_name":"Human Toll-like receptor 2 / TLR2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d0589835-e5e0-5002-80eb-a5d056fd57f9","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-yrs-tlr2-event","event_type":"observed_relationship","label":"A protein used for translation also signals outside cells.","description":"Extracellular YARS1 activated TLR2 signaling and inflammatory mediator release in human monocyte/macrophage preparations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1e70f8cb-144f-5c63-b862-0c8670840077","slug":"yars1","display_name":"Human cytosolic tyrosyl-tRNA synthetase / YARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7d88fac7-eee3-541d-a202-e73c3f04186e","slug":"tlr2","display_name":"Human Toll-like receptor 2 / TLR2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"875a94da-1eee-50d2-b5c1-e4316c2a8f45","slug":"nf-kappab-signaling","display_name":"NF-kappa-B signaling","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text and abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"THP1 and peripheral-blood-derived macrophages; purified protein exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This differs from intracellular charging and from assays of individual fragments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A protein used for translation also signals outside cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Moonlighting matrix metalloproteinase substrates: Enhancement of proinflammatory functions of extracellular tyrosyl-tRNA synthetase upon cleavage. · 2020 · https://pubmed.ncbi.nlm.nih.gov/31771979/ · DOI 10.1074/jbc.RA119.010486","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1469aecc-2679-5207-85b7-9508de3cdbb1","evidence_kind":"source_excerpt","locator":"Lines 108-114","start_line":108,"end_line":114,"excerpt":"## l-tyrosine-yrs-tlr2\nA protein used for translation also signals outside cells.\nExtracellular YARS1 activated TLR2 signaling and inflammatory mediator release in human monocyte/macrophage preparations.\nModel: THP1 and peripheral-blood-derived macrophages; purified protein exposure.\nLimitations: This differs from intracellular charging and from assays of individual fragments.\nEvidence access: Primary full text and abstract\nMoonlighting matrix metalloproteinase substrates: Enhancement of proinflammatory functions of extracellular tyrosyl-tRNA synthetase upon cleavage. · 2020 · https://pubmed.ncbi.nlm.nih.gov/31771979/ · DOI 10.1074/jbc.RA119.010486","model_system":"THP1 and peripheral-blood-derived macrophages; purified protein exposure.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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