{"id":"73cf55b7-8a59-5142-bed1-cb9a7c586855","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-lipid-peroxide-radicals","predicate":"generates","statement":"V(IV) generated by ascorbate/vanadate chemistry produced hydroperoxide-derived radicals from cumene hydroperoxide.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c68f87fb-1df0-5064-bc64-35fbf3c4e58a","mechanism_event_label":"The same redox system can also react with a model lipid peroxide.","subject":{"id":"656eb223-933b-5a40-b70c-0d77537fe717","slug":"vanadyl-iv","display_name":"Oxovanadium(IV) / vanadyl","entity_type_key":"ion"},"object":{"id":"12a8191c-029f-59de-a23c-e41014e1bb8d","slug":"vanadium-lipid-hydroperoxide-derived-radicals","display_name":"Radicals derived from hydroperoxide in vanadium assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c68f87fb-1df0-5064-bc64-35fbf3c4e58a","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-lipid-peroxide-radicals-event","event_type":"observed_relationship","label":"The same redox system can also react with a model lipid peroxide.","description":"V(IV) generated by ascorbate/vanadate chemistry produced hydroperoxide-derived radicals from cumene hydroperoxide.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"656eb223-933b-5a40-b70c-0d77537fe717","slug":"vanadyl-iv","display_name":"Oxovanadium(IV) / vanadyl","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"12a8191c-029f-59de-a23c-e41014e1bb8d","slug":"vanadium-lipid-hydroperoxide-derived-radicals","display_name":"Radicals derived from hydroperoxide in vanadium assays","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b28dfc54-a5ef-5f9e-ac1f-890b68e58dc3","slug":"inorganic-phosphate","display_name":"Inorganic phosphate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free model-hydroperoxide assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cumene hydroperoxide is a model reagent, not direct measurement of membrane injury in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The same redox system can also react with a model lipid peroxide.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"One-electron reduction of vanadate by ascorbate and related free radical generation at physiological pH. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8051539/ · DOI 10.1016/0162-0134(94)85032-1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1e7ba1f5-382c-55d2-8a46-a0a311fcbcb8","evidence_kind":"source_excerpt","locator":"Lines 110-116","start_line":110,"end_line":116,"excerpt":"## vanadium-lipid-peroxide-radicals\nThe same redox system can also react with a model lipid peroxide.\nV(IV) generated by ascorbate/vanadate chemistry produced hydroperoxide-derived radicals from cumene hydroperoxide.\nModel: Cell-free model-hydroperoxide assay.\nLimitations: Cumene hydroperoxide is a model reagent, not direct measurement of membrane injury in humans.\nEvidence access: Primary abstract\nOne-electron reduction of vanadate by ascorbate and related free radical generation at physiological pH. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8051539/ · DOI 10.1016/0162-0134(94)85032-1","model_system":"Cell-free model-hydroperoxide assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"d36b30d972e756ca4d19b66f976980e206c65025bd4bbf4c127c17320bd019c2","revision_id":"70d42967-8032-5627-8670-f59841735e89","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}