{"id":"73b368d9-737c-5bb6-87c1-bfb957051981","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq3-sam","predicate":"uses","statement":"COQ3 methylated the tested precursor in the presence of SAM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9fe07584-f875-5ac1-849b-4182ab24c557","mechanism_event_label":"CoQ synthesis shares the methyl-donor pool used by other pathways.","subject":{"id":"1ef33c17-ea2a-5690-a325-711e93d0da58","slug":"tet-coq3","display_name":"Reconstructed ancestral tetrapod COQ3","entity_type_key":"protein"},"object":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"9fe07584-f875-5ac1-849b-4182ab24c557","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq3-sam-event","event_type":"biochemical_relationship","label":"CoQ synthesis shares the methyl-donor pool used by other pathways.","description":"COQ3 methylated the tested precursor in the presence of SAM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f71dde75-96d5-5fac-84f7-e9675f8e31dd","slug":"coq-o-methylation","display_name":"Coenzyme Q precursor O-methylation","entity_type_key":"cellular_process"},"role":"reaction","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1ef33c17-ea2a-5690-a325-711e93d0da58","slug":"tet-coq3","display_name":"Reconstructed ancestral tetrapod COQ3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 13814, \"end_char\": 15577, \"text_sha256\": \"c43de1ac5dc6711fad8e37af3a52a8cd9784ed7eeaf77bd2981afe72abaf307f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified reconstructed COQ metabolon with short-chain substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Enzyme combinations, methyl donors, reductants and metal additions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Reconstructed ancestral tetrapod proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CoQ synthesis shares the methyl-donor pool used by other pathways.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Stepwise CoQ head-group assembly","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"95b5a97c-c827-5394-9ef9-ec5a589fe9d5","evidence_kind":"source_excerpt","locator":"Lines 645-656","start_line":645,"end_line":656,"excerpt":"### coq10-coq3-sam\nCOQ3 methylated the tested precursor in the presence of SAM.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CoQ synthesis shares the methyl-donor pool used by other pathways.\norganism: Reconstructed ancestral tetrapod proteins\ntissue_or_cell_type: Stepwise CoQ head-group assembly\nexperimental_model: Purified reconstructed COQ metabolon with short-chain substrates\nlimitations: Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.\nexposure: Enzyme combinations, methyl donors, reductants and metal additions\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 13814, \"end_char\": 15577, \"text_sha256\": \"c43de1ac5dc6711fad8e37af3a52a8cd9784ed7eeaf77bd2981afe72abaf307f\"}\n[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","model_system":"Purified reconstructed COQ metabolon with short-chain substrates","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fc6c2f3b-14a3-59de-848e-ca1c02fee1df","stable_key":"import-3b5aff9b-4086-5574-bfb4-3ea49ba520d7","title":"Coenzyme Q10: biosynthesis, electron transfer, antioxidant recycling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"053f9a6f6c17321fa9fd271486d7f20a86a49b237424108de7dc8663372e73bf","revision_id":"028900ce-8ca4-5d13-8c86-6e327c071e64","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}