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(2022). https://pmc.ncbi.nlm.nih.gov/articles/PMC11072207/ DOI: 10.1007/s00018-022-04404-0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skeletal muscle and embryonic mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"cec95740-e104-53a5-adc5-f5d2844696a7","evidence_kind":"source_excerpt","locator":"Lines 490-501","start_line":490,"end_line":501,"excerpt":"### transport-slc25a32-fad-import\nK235R/K235R and Y174C/K235R mouse muscle mitochondria accumulated less isotope-labeled FAD; Slc25a32-null embryonic mitochondria also had impaired FAD uptake.\nCondition category: machinery_impairment\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The carrier defect restricts access to mitochondrial FAD.\norganism: Mus musculus\ntissue_or_cell_type: Skeletal muscle and embryonic mitochondria\nexperimental_model: Stable-isotope uptake in isolated knock-in/knockout mitochondria\nlimitations: Organelle uptake supports carrier dependence but does not establish exchange partner or transport stoichiometry.\nexposure: 10 micromolar isotope-labeled FAD; knockout embryo endpoint at 60 minutes.\nevidence_location: Figure 5C-D\n[transport-slc25a32-2022] Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. 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