{"id":"73710a8b-b490-50d6-acd3-17702d5c9af8","stable_key":"fe6af7fc-7372-5c4f-9c2f-2bbf56695397:phosphorus-casr-r62","predicate":"loses_inhibition_by","statement":"The R62A CaSR construct lost the phosphate-induced receptor inhibition observed with wild-type receptor.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ad9820bf-6537-5fdf-9da4-a56bb11a8711","mechanism_event_label":"Altering a specific receptor site removed the phosphate response.","subject":{"id":"43e36efa-3143-57af-8fc0-4fc6f2852fa6","slug":"casr-r62a","display_name":"Human CaSR R62A construct","entity_type_key":"protein_state"},"object":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"ad9820bf-6537-5fdf-9da4-a56bb11a8711","stable_key":"fe6af7fc-7372-5c4f-9c2f-2bbf56695397:phosphorus-casr-r62-event","event_type":"biochemical_relationship","label":"Altering a specific receptor site removed the phosphate response.","description":"The R62A CaSR construct lost the phosphate-induced receptor inhibition observed with wild-type receptor.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"43e36efa-3143-57af-8fc0-4fc6f2852fa6","slug":"casr-r62a","display_name":"Human CaSR R62A construct","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/phosphorus-research/31619668.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e4fe1ed7eb92175f99f446afa19fb184147ceed0413f3a6dffa85aa80927015d\", \"start_char\": 0, \"end_char\": 1144, \"text_sha256\": \"e4fe1ed7eb92175f99f446afa19fb184147ceed0413f3a6dffa85aa80927015d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"CaSR expression/mutagenesis and isolated human/mouse parathyroid experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Extracellular phosphate in pathophysiologic CKD ranges; R62A substitution and mouse Casr deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"R62A identity resolved in full text; not a common variant or a dietary model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Phosphorus research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"phosphorus","display_name":"Phosphorus","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human CaSR construct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Altering a specific receptor site removed the phosphate response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[phosphorus-p31619668] Phosphate acts directly on the calcium-sensing receptor to stimulate parathyroid hormone secretion. 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