{"id":"736ee1ee-f03d-59db-99bf-c6303dc2eb0a","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-mouse-cartilage","predicate":"suppresses","statement":"Ergothioneine treatment reduced inflammatory and matrix-degrading responses in IL-1beta-stimulated mouse chondrocytes through a reported Sirt6/NF-kappaB axis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f876834d-e1ac-5d61-870d-6663646f5d0a","mechanism_event_label":"Cartilage cells provide another signaling context.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"16f1f594-0a81-5596-a852-9c49aee7ced8","slug":"mouse-chondrocyte-nfkb-inflammation","display_name":"Mouse chondrocyte NF-kappaB-associated inflammation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f876834d-e1ac-5d61-870d-6663646f5d0a","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-mouse-cartilage-event","event_type":"observed_relationship","label":"Cartilage cells provide another signaling context.","description":"Ergothioneine treatment reduced inflammatory and matrix-degrading responses in IL-1beta-stimulated mouse chondrocytes through a reported Sirt6/NF-kappaB axis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"16f1f594-0a81-5596-a852-9c49aee7ced8","slug":"mouse-chondrocyte-nfkb-inflammation","display_name":"Mouse chondrocyte NF-kappaB-associated inflammation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3fa7b3a0-1f27-583a-995e-601852f03722","slug":"mouse-sirt6","display_name":"Mouse sirtuin 6 / Sirt6","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"875a94da-1eee-50d2-b5c1-e4316c2a8f45","slug":"nf-kappab-signaling","display_name":"NF-kappa-B signaling","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse chondrocytes; 10 ng/mL IL-1beta; DMM mouse OA model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not human osteoarthritis treatment evidence; direct Sirt6 binding is not established by this abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Cartilage cells provide another signaling context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ergothioneine inhibits the progression of osteoarthritis via the Sirt6/NF-κB axis both in vitro and in vivo. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37156032/ · DOI 10.1016/j.intimp.2023.110211","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"50f89fa5-3ea1-530a-a191-f7a266d67652","evidence_kind":"source_excerpt","locator":"Lines 368-374","start_line":368,"end_line":374,"excerpt":"## ergothioneine-mouse-cartilage\nCartilage cells provide another signaling context.\nErgothioneine treatment reduced inflammatory and matrix-degrading responses in IL-1beta-stimulated mouse chondrocytes through a reported Sirt6/NF-kappaB axis.\nModel: Mouse chondrocytes; 10 ng/mL IL-1beta; DMM mouse OA model.\nLimitations: Not human osteoarthritis treatment evidence; direct Sirt6 binding is not established by this abstract.\nEvidence access: Primary abstract\nErgothioneine inhibits the progression of osteoarthritis via the Sirt6/NF-κB axis both in vitro and in vivo. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37156032/ · DOI 10.1016/j.intimp.2023.110211","model_system":"Mouse chondrocytes; 10 ng/mL IL-1beta; DMM mouse OA model.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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