{"id":"735d5165-d08e-5ed8-8473-4315627e3533","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:pparg-dependence-protocatechuic-acid","predicate":"decreases_in_recorded_experiment","statement":"PPARgamma inhibition or silencing blocked the polyphenol-associated GLUT4 recruitment in mouse 3T3-L1 adipocytes.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"87ff1392-265b-531d-ac31-6c77c73024b5","mechanism_event_label":"PPARgamma inhibition or silencing blocked the polyphenol-associated GLUT4 recruitment in mouse 3T3-L1 adipocytes.","subject":{"id":"f7042803-09ec-53d9-9d4c-9a0ce4042c00","slug":"protocatechuic-acid","display_name":"Protocatechuic acid / 3,4-dihydroxybenzoic acid","entity_type_key":"small_molecule"},"object":{"id":"708057bc-62a1-5796-8c1c-39b03fe230d0","slug":"mouse-glut4-surface-translocation","display_name":"Mouse GLUT4 movement toward or into the cell surface","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"87ff1392-265b-531d-ac31-6c77c73024b5","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:pparg-dependence-protocatechuic-acid-event","event_type":"experimental_observation","label":"PPARgamma inhibition or silencing blocked the polyphenol-associated GLUT4 recruitment in mouse 3T3-L1 adipocytes.","description":"**Adipocyte glucose uptake involves PPARγ in one high-exposure model.** Human omental adipocytes exposed to 50 µM C3G or 100 µM PCA showed increased glucose uptake, GLUT4 recruitment and adiponectin-related responses. The decisive PPARγ silencing and antagonist experiments were performed in mouse 3T3-L1 adipocytes: disrupting PPARγ prevented polyphenol-associated GLUT4/adiponectin upregulation and GLUT4 recruitment. These mouse perturbations must not be relabeled human knockdowns. The human-cell concentrations are far above the selected free serum peaks in the tracer study; the experiment does not establish an insulin-like dietary effect. [Scazzocchio et al., 2011](https://pmc.ncbi.nlm.nih.gov/articles/PMC3161313/).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f7042803-09ec-53d9-9d4c-9a0ce4042c00","slug":"protocatechuic-acid","display_name":"Protocatechuic acid / 3,4-dihydroxybenzoic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"protocatechuic-acid with mouse Pparg inhibition/silencing","sequence_order":0,"notes":""},{"entity":{"id":"708057bc-62a1-5796-8c1c-39b03fe230d0","slug":"mouse-glut4-surface-translocation","display_name":"Mouse GLUT4 movement toward or into the cell surface","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"8e9622fd-f3a4-526b-9ab5-7389d768d937","slug":"mouse-pparg","display_name":"Mouse peroxisome proliferator-activated receptor gamma / Pparg","entity_type_key":"protein"},"role":"experimentally inhibited regulatory factor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4ee1fc5c-bc41-5de5-a56e-50ec13fe736a","slug":"mouse-adipoq","display_name":"Mouse adiponectin / Adipoq","entity_type_key":"protein"},"role":"additional transcript output in study","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"66d34dc0-d0fc-5ebb-9d7c-dc88f6b47cea","slug":"mouse-slc2a4","display_name":"Mouse glucose transporter 4 / Slc2a4","entity_type_key":"protein"},"role":"regulated transporter","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Full text retrieved; relevant methods/results/figure text reviewed. 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Supports pathway involvement, not direct binding affinity or clinical supplementation efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PPARgamma inhibition or silencing blocked the polyphenol-associated GLUT4 recruitment in mouse 3T3-L1 adipocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cyanidin-3-O-β-glucoside and protocatechuic acid exert insulin-like effects by upregulating PPARγ activity in human omental adipocytes. | 2011 | DOI 10.2337/db10-1461 | PMID 21788573 | https://pubmed.ncbi.nlm.nih.gov/21788573/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC3161313/ | https://doi.org/10.2337/db10-1461","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 70-70; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1973bcfc-0c63-5ee0-a0c7-58eca9cb735f","evidence_kind":"source_excerpt","locator":"Lines 70-70","start_line":70,"end_line":70,"excerpt":"**Adipocyte glucose uptake involves PPARγ in one high-exposure model.** Human omental adipocytes exposed to 50 µM C3G or 100 µM PCA showed increased glucose uptake, GLUT4 recruitment and adiponectin-related responses. The decisive PPARγ silencing and antagonist experiments were performed in mouse 3T3-L1 adipocytes: disrupting PPARγ prevented polyphenol-associated GLUT4/adiponectin upregulation and GLUT4 recruitment. These mouse perturbations must not be relabeled human knockdowns. The human-cell concentrations are far above the selected free serum peaks in the tracer study; the experiment does not establish an insulin-like dietary effect. [Scazzocchio et al., 2011](https://pmc.ncbi.nlm.nih.gov/articles/PMC3161313/).","model_system":"Mouse 3T3-L1, Figure 7: mouse Pparg siRNA/scrambled comparison and GW9662 antagonist; 10 uM C3G or 100 uM PCA.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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