{"id":"7353e753-68f8-5933-a4f2-5f83acdfb8a8","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-bco2-13cis","predicate":"cleaves_to","statement":"Ferret BCO2 also cleaved 13-cis lycopene to apo-10-prime-lycopenal.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bcd75bad-8882-5603-bfab-bf363d5ed174","mechanism_event_label":"Another cis form entered the same measured cleavage route.","subject":{"id":"cf0a614d-af35-5332-880a-f92c9466a294","slug":"ferret-bco2","display_name":"Ferret beta-carotene oxygenase 2 / BCO2","entity_type_key":"protein"},"object":{"id":"00f65e41-237c-5844-bafe-0daf6ee73207","slug":"apo-10-lycopenal","display_name":"Apo-10-prime-lycopenal","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"bcd75bad-8882-5603-bfab-bf363d5ed174","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-bco2-13cis-event","event_type":"biochemical_relationship","label":"Another cis form entered the same measured cleavage route.","description":"Ferret BCO2 also cleaved 13-cis lycopene to apo-10-prime-lycopenal.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fe8adab6-87df-549e-97c6-b6aa1587c4b7","slug":"13-cis-lycopene","display_name":"13-cis-Lycopene","entity_type_key":"small_molecule"},"role":"tested_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cf0a614d-af35-5332-880a-f92c9466a294","slug":"ferret-bco2","display_name":"Ferret beta-carotene oxygenase 2 / BCO2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"00f65e41-237c-5844-bafe-0daf6ee73207","slug":"apo-10-lycopenal","display_name":"Apo-10-prime-lycopenal","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/16672231.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"135695c9d3106469d8d64ec0806ba5bf048f0f1df6f3e3cd53e838e50b960445\", \"start_char\": 0, \"end_char\": 1967, \"text_sha256\": \"135695c9d3106469d8d64ec0806ba5bf048f0f1df6f3e3cd53e838e50b960445\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant ferret enzyme and tissue-fraction metabolism","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"5-cis, 13-cis and all-trans lycopene; iron; NAD+ or NADH","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ferret experiments do not quantify human cleavage flux or predict supplement response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Ferret","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Another cis form entered the same measured cleavage route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p16672231] The biochemical characterization of ferret carotene-9',10'-monooxygenase catalyzing cleavage of carotenoids in vitro and in vivo. (2006). https://pubmed.ncbi.nlm.nih.gov/16672231/ DOI: 10.1074/jbc.m512095200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"BCO2 enzyme; hepatic fractions and lung","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"faab1052-9079-50e4-90dc-d8b17235b6b7","evidence_kind":"source_excerpt","locator":"Lines 299-310","start_line":299,"end_line":310,"excerpt":"### lycopene-bco2-13cis\nFerret BCO2 also cleaved 13-cis lycopene to apo-10-prime-lycopenal.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Another cis form entered the same measured cleavage route.\norganism: Ferret\ntissue_or_cell_type: BCO2 enzyme; hepatic fractions and lung\nexperimental_model: Recombinant ferret enzyme and tissue-fraction metabolism\nlimitations: Ferret experiments do not quantify human cleavage flux or predict supplement response.\nexposure: 5-cis, 13-cis and all-trans lycopene; iron; NAD+ or NADH\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/16672231.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"135695c9d3106469d8d64ec0806ba5bf048f0f1df6f3e3cd53e838e50b960445\", \"start_char\": 0, \"end_char\": 1967, \"text_sha256\": \"135695c9d3106469d8d64ec0806ba5bf048f0f1df6f3e3cd53e838e50b960445\"}\n[lycopene-p16672231] The biochemical characterization of ferret carotene-9',10'-monooxygenase catalyzing cleavage of carotenoids in vitro and in vivo. 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