{"id":"73429c75-2c30-51bc-99d4-02dc1643387c","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-leydig-trafficking","predicate":"attenuates_tested","statement":"D-aspartate attenuated hCG-associated LHR staining redistribution from membrane to cytoplasm without changing total LHR protein.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6a539fb8-22e3-575f-9640-89a69547c6cc","mechanism_event_label":"Receptor location changed even when total receptor amount did not.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"58fe161f-5c66-50e4-a1bf-4061702f5d77","slug":"mouse-lhcgr-internalization","display_name":"LHCGR membrane-to-cytoplasm redistribution in mouse MA-10 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6a539fb8-22e3-575f-9640-89a69547c6cc","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-leydig-trafficking-event","event_type":"observed_relationship","label":"Receptor location changed even when total receptor amount did not.","description":"D-aspartate attenuated hCG-associated LHR staining redistribution from membrane to cytoplasm without changing total LHR protein.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58fe161f-5c66-50e4-a1bf-4061702f5d77","slug":"mouse-lhcgr-internalization","display_name":"LHCGR membrane-to-cytoplasm redistribution in mouse MA-10 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6ee453fb-ad7f-54ca-ab03-6397b781901e","slug":"human-chorionic-gonadotropin","display_name":"Human chorionic gonadotropin","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ab131b98-fb5f-5dca-b5de-92898e4cf18f","slug":"mouse-lhcgr","display_name":"Mouse luteinizing-hormone/choriogonadotropin receptor / Lhcgr","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse MA-10 immunofluorescence and immunoblot comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Supports a trafficking hypothesis; not proof of universally delayed receptor internalization in human testes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Receptor location changed even when total receptor amount did not.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"D-Aspartic acid stimulates steroidogenesis through the delay of LH receptor internalization in a mammalian Leydig cell line. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26122485/ · DOI 10.1007/s40618-015-0333-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9d96ed72-611a-54b9-8476-04089ebe14b9","evidence_kind":"source_excerpt","locator":"Lines 400-406","start_line":400,"end_line":406,"excerpt":"## d-aspartate-leydig-trafficking\nReceptor location changed even when total receptor amount did not.\nD-aspartate attenuated hCG-associated LHR staining redistribution from membrane to cytoplasm without changing total LHR protein.\nModel: Mouse MA-10 immunofluorescence and immunoblot comparison.\nLimitations: Supports a trafficking hypothesis; not proof of universally delayed receptor internalization in human testes.\nEvidence access: Primary abstract\nD-Aspartic acid stimulates steroidogenesis through the delay of LH receptor internalization in a mammalian Leydig cell line. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26122485/ · DOI 10.1007/s40618-015-0333-4","model_system":"Mouse MA-10 immunofluorescence and immunoblot comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0a86a1008edb8a69dcc79409e76e3fd5ce59b0747be8cae8f21e43f871e76248","revision_id":"a1de4b66-263d-5452-8ed8-df853afba030","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}