{"id":"733cc923-9c2c-5255-b8f3-9fdf792a2564","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-casr-knockout-erk","predicate":"deletion_prevents","statement":"The reported Casr knockout abolished rapid strontium-associated ERK1/2 phosphorylation in primary mouse osteoblasts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"65004064-b115-52bb-9009-27af0428e7a8","mechanism_event_label":"One signaling branch requires the receptor in this model.","subject":{"id":"18071fe3-a86f-5dac-b440-1e2e59d22f24","slug":"mouse-casr","display_name":"Mouse calcium-sensing receptor / Casr","entity_type_key":"protein"},"object":{"id":"63aa8dd5-93d8-506e-9a1b-d6057f776e4a","slug":"mouse-strontium-erk-phosphorylation","display_name":"Rapid ERK phosphorylation after strontium in mouse osteoblasts","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"65004064-b115-52bb-9009-27af0428e7a8","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-casr-knockout-erk-event","event_type":"observed_relationship","label":"One signaling branch requires the receptor in this model.","description":"The reported Casr knockout abolished rapid strontium-associated ERK1/2 phosphorylation in primary mouse osteoblasts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"18071fe3-a86f-5dac-b440-1e2e59d22f24","slug":"mouse-casr","display_name":"Mouse calcium-sensing receptor / Casr","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"63aa8dd5-93d8-506e-9a1b-d6057f776e4a","slug":"mouse-strontium-erk-phosphorylation","display_name":"Rapid ERK phosphorylation after strontium in mouse osteoblasts","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text, publicly accessible Methods and results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse calvarial cells; exon-4-disrupted Casr model; SrCl2/sodium-ranelate mixture at 100:1, expressed as Sr2+ concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Genotype and assay-specific finding; other response branches persisted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"One signaling branch requires the receptor in this model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Calcium sensing receptor-dependent and receptor-independent activation of osteoblast replication and survival by strontium ranelate. · 2009 · https://pubmed.ncbi.nlm.nih.gov/20141614/ · DOI 10.1111/j.1582-4934.2009.00673.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"5a649206-3f1b-5939-be6e-ab9957a96cfc","evidence_kind":"source_excerpt","locator":"Lines 86-92","start_line":86,"end_line":92,"excerpt":"## strontium-casr-knockout-erk\nOne signaling branch requires the receptor in this model.\nThe reported Casr knockout abolished rapid strontium-associated ERK1/2 phosphorylation in primary mouse osteoblasts.\nModel: Mouse calvarial cells; exon-4-disrupted Casr model; SrCl2/sodium-ranelate mixture at 100:1, expressed as Sr2+ concentration.\nLimitations: Genotype and assay-specific finding; other response branches persisted.\nEvidence access: Primary full text, publicly accessible Methods and results\nCalcium sensing receptor-dependent and receptor-independent activation of osteoblast replication and survival by strontium ranelate. · 2009 · https://pubmed.ncbi.nlm.nih.gov/20141614/ · DOI 10.1111/j.1582-4934.2009.00673.x","model_system":"Mouse calvarial cells; exon-4-disrupted Casr model; SrCl2/sodium-ranelate mixture at 100:1, expressed as Sr2+ concentration.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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