{"id":"733c8863-6c93-5f42-b47d-511c2308d73c","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:3typ-acetylation","predicate":"increases","statement":"3-TYP treatment increased mitochondrial protein acetylation in neonatal rat cardiomyocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7afb3f79-dfd3-5cae-bcde-34c2d1456c63","mechanism_event_label":"3typ acetylation","subject":{"id":"0769c420-b827-5567-986f-19b0c2ecefd6","slug":"3-typ","display_name":"3-TYP experimental SIRT3 inhibitor","entity_type_key":"drug"},"object":{"id":"7268d68c-125d-5d24-9d55-a2508f6c0031","slug":"rat-mitochondrial-protein-acetylation","display_name":"Rat cardiac mitochondrial protein lysine acetylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7afb3f79-dfd3-5cae-bcde-34c2d1456c63","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:3typ-acetylation","event_type":"increases","label":"3typ acetylation","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0769c420-b827-5567-986f-19b0c2ecefd6","slug":"3-typ","display_name":"3-TYP experimental SIRT3 inhibitor","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7268d68c-125d-5d24-9d55-a2508f6c0031","slug":"rat-mitochondrial-protein-acetylation","display_name":"Rat cardiac mitochondrial protein lysine acetylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c8e2702d-f4d3-5d3e-a2a4-4bf7fef91a2c","slug":"rat-cardiac-sirt3","display_name":"Rat cardiac sirtuin 3 / Sirt3","entity_type_key":"protein"},"role":"intended pharmacological target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Compound concentration not established in reviewed text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Treatment endpoint timing not established in reviewed text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"rat-mitochondrial-protein-acetylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 2A","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pharmacological 3-TYP experiment; NR co-treatment where stated","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"3-typ","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. 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Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Pharmacological inhibition is not a Sirt3 genetic-specificity experiment; concentration not specified in reviewed text.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 23, \"text_sha256\": \"08f11746524e975c9e1a5e3d53de363a6762891bc05afffbd711f347867ac842\", \"xml_element_id\": \"Par10\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 25, \"text_sha256\": \"1814242d6194ed4e0b3ab78287e20f85b6bc243051183d0f24d9034c3a930257\", \"xml_element_id\": null}]\nplain_language: 3-TYP treatment increased mitochondrial protein acetylation in neonatal rat cardiomyocytes.","model_system":"Pharmacological 3-TYP experiment; NR co-treatment where stated","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}