{"id":"72faca30-d747-5963-a385-ca5ce2b75ffc","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-monascin-glucosidase","predicate":"inhibits_tested","statement":"Monascin inhibited yeast alpha-glucosidase with an IC50 of 302.6 micromolar and mixed-type kinetics.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"373638e6-e9bc-59a7-a066-027045220e05","mechanism_event_label":"The isolated pigment inhibited a carbohydrate-cleaving enzyme preparation.","subject":{"id":"f30a9f70-f7e3-523a-8948-5aff9593eacd","slug":"monascin","display_name":"Monascin","entity_type_key":"small_molecule"},"object":{"id":"d40ae172-7698-5aaa-8917-aba671505cc9","slug":"yeast-alpha-glucosidase-ryr-assay","display_name":"Saccharomyces cerevisiae alpha-glucosidase in the 2024 pigment assay","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"373638e6-e9bc-59a7-a066-027045220e05","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-monascin-glucosidase-event","event_type":"observed_relationship","label":"The isolated pigment inhibited a carbohydrate-cleaving enzyme preparation.","description":"Monascin inhibited yeast alpha-glucosidase with an IC50 of 302.6 micromolar and mixed-type kinetics.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f30a9f70-f7e3-523a-8948-5aff9593eacd","slug":"monascin","display_name":"Monascin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d40ae172-7698-5aaa-8917-aba671505cc9","slug":"yeast-alpha-glucosidase-ryr-assay","display_name":"Saccharomyces cerevisiae alpha-glucosidase in the 2024 pigment assay","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text PMC11120408","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Saccharomyces cerevisiae enzyme, pNPG substrate, pH 6.8; one-hour preincubation and 30-minute reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Yeast enzyme, not human intestinal maltase-glucoamylase or sucrase-isomaltase; potency is not clinical glucose lowering.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Red yeast rice collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"red-yeast-rice","display_name":"Red yeast rice","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The isolated pigment inhibited a carbohydrate-cleaving enzyme preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[38790873] Revealing the Hypoglycemic Effect of Red Yeast Rice: Perspectives from the Inhibition of α-Glucosidase and the Anti-Glycation Capability by Ankaflavin and Monascin. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38790873/ · DOI 10.3390/foods13101573","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"661daac9-36ff-57ec-9843-fb801539e916","evidence_kind":"source_excerpt","locator":"Lines 292-298","start_line":292,"end_line":298,"excerpt":"## red-yeast-rice-monascin-glucosidase\nThe isolated pigment inhibited a carbohydrate-cleaving enzyme preparation.\nMonascin inhibited yeast alpha-glucosidase with an IC50 of 302.6 micromolar and mixed-type kinetics.\nModel: Saccharomyces cerevisiae enzyme, pNPG substrate, pH 6.8; one-hour preincubation and 30-minute reaction.\nLimitations: Yeast enzyme, not human intestinal maltase-glucoamylase or sucrase-isomaltase; potency is not clinical glucose lowering.\nEvidence access: Primary full text PMC11120408\n[38790873] Revealing the Hypoglycemic Effect of Red Yeast Rice: Perspectives from the Inhibition of α-Glucosidase and the Anti-Glycation Capability by Ankaflavin and Monascin. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38790873/ · DOI 10.3390/foods13101573","model_system":"Saccharomyces cerevisiae enzyme, pNPG substrate, pH 6.8; one-hour preincubation and 30-minute reaction.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"261a12bc-3d00-52a4-a5ec-70e174390596","stable_key":"import-4910e17b-aaec-52cd-b9e5-caca85d817ee","title":"Red yeast rice: constituents, mevalonate, CoQ and product-specific interactions (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"271bbcecaaacc76ad4d06718ae592dc6a1b33e3e459adfdd36cbd0ac5f09c0f6","revision_id":"9c30956c-206c-5c49-ae42-81b070088e26","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}