{"id":"72e8663f-5401-5bb4-8f64-8522b10778a2","stable_key":"53d9b98d-e3a0-5416-bc88-adbe42a8cb1b:curcumin-nac-buffering","predicate":"reduces","statement":"NAC pretreatment shifted the curcumin dose-response curve toward lower inhibitory potency in RAW264.7 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0a49b080-27f6-56c9-98e7-b25677126932","mechanism_event_label":"A glutathione precursor changed the response in the opposite direction from blocking glutathione synthesis.","subject":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"object":{"id":"789eec88-fc94-51d3-a067-02fa4cfe105f","slug":"curcumin-nfkb-inhibition","display_name":"Curcumin-associated inhibition of stimulated NF-kappa-B activity in the tested cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0a49b080-27f6-56c9-98e7-b25677126932","stable_key":"53d9b98d-e3a0-5416-bc88-adbe42a8cb1b:curcumin-nac-buffering-event","event_type":"biochemical_relationship","label":"A glutathione precursor changed the response in the opposite direction from blocking glutathione synthesis.","description":"NAC pretreatment shifted the curcumin dose-response curve toward lower inhibitory potency in RAW264.7 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0e625439-d093-5d1b-8713-dcfdafcf6811","slug":"curcumin","display_name":"Curcumin","entity_type_key":"small_molecule"},"role":"tested_compound","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"proposed_buffering_pathway","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"789eec88-fc94-51d3-a067-02fa4cfe105f","slug":"curcumin-nfkb-inhibition","display_name":"Curcumin-associated inhibition of stimulated NF-kappa-B activity in the tested cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/curcumin-research/29097552.publisher-preview.txt\", \"locator\": \"Primary public publisher preview, reference superscripts removed; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"fe26fe4a94e0ab5b60d128b86deb5bc8723e13a0403fcbdba7ef97224a22ef9a\", \"start_char\": 0, \"end_char\": 2711, \"text_sha256\": \"fe26fe4a94e0ab5b60d128b86deb5bc8723e13a0403fcbdba7ef97224a22ef9a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Curcumin analog chemistry and stimulated cellular NF-kappa-B assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"NAC 3 mM overnight before the curcumin/LPS reporter assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell assay, not evidence that oral NAC cancels curcumin benefits in people; enhanced glutathione buffering was the authors proposed this explanation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Curcumin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"curcumin","display_name":"Curcumin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A glutathione precursor changed the response in the opposite direction from blocking glutathione synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[curcumin-p29097552] The anti-inflammatory activity of curcumin is mediated by its oxidative metabolites. (2017). https://pubmed.ncbi.nlm.nih.gov/29097552/ DOI: 10.1074/jbc.ra117.000123","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"RAW264.7 macrophage-like cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6587f1cf-0caf-5d1c-ad49-898315ea47bf","evidence_kind":"source_excerpt","locator":"Lines 489-500","start_line":489,"end_line":500,"excerpt":"### curcumin-nac-buffering\nNAC pretreatment shifted the curcumin dose-response curve toward lower inhibitory potency in RAW264.7 cells.\nCondition category: normal\nnutrient_topic: Curcumin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A glutathione precursor changed the response in the opposite direction from blocking glutathione synthesis.\norganism: Mus musculus\ntissue_or_cell_type: RAW264.7 macrophage-like cells\nexperimental_model: Curcumin analog chemistry and stimulated cellular NF-kappa-B assays\nlimitations: Cell assay, not evidence that oral NAC cancels curcumin benefits in people; enhanced glutathione buffering was the authors proposed this explanation.\nexposure: NAC 3 mM overnight before the curcumin/LPS reporter assay\nevidence_span: {\"source_cache\": \"artifacts/curcumin-research/29097552.publisher-preview.txt\", \"locator\": \"Primary public publisher preview, reference superscripts removed; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"fe26fe4a94e0ab5b60d128b86deb5bc8723e13a0403fcbdba7ef97224a22ef9a\", \"start_char\": 0, \"end_char\": 2711, \"text_sha256\": \"fe26fe4a94e0ab5b60d128b86deb5bc8723e13a0403fcbdba7ef97224a22ef9a\"}\n[curcumin-p29097552] The anti-inflammatory activity of curcumin is mediated by its oxidative metabolites. (2017). https://pubmed.ncbi.nlm.nih.gov/29097552/ DOI: 10.1074/jbc.ra117.000123","model_system":"Curcumin analog chemistry and stimulated cellular NF-kappa-B assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [curcumin-p29097552] The anti-inflammatory activity of curcumin is mediated by its oxidative metabolites. (2017). https://pubmed.ncbi.nlm.nih.gov/29097552/ DOI: 10.1074/jbc.ra117.000123","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"11d29ea9-3af1-5404-a68d-6f129e23edee","stable_key":"import-53d9b98d-e3a0-5416-bc88-adbe42a8cb1b","title":"Curcumin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"6eeda0e6e358e0b19f73f554f8c027cb08015aa49d92a77bde36e3fb3ea1a41c","revision_id":"32e73d69-5445-5ace-b25d-5a4d3787aea5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}