{"id":"72de0155-ae5a-5945-ba78-ed9849a4bfdc","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-pig-sodium-uptake","predicate":"supports","statement":"Ex vivo pig jejunal L-threonine flux was sodium dependent in assays using 50 micromolar and 5 millimolar amino-acid concentrations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"71f463d7-1401-5c17-ba10-d73a40ace765","mechanism_event_label":"An ion gradient contributes to intestinal amino-acid movement.","subject":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"object":{"id":"57e6f495-f432-5a4b-bfef-35af3d9dd423","slug":"pig-jejunal-threonine-flux","display_name":"Pig jejunal mucosal-to-serosal threonine flux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"71f463d7-1401-5c17-ba10-d73a40ace765","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-pig-sodium-uptake-event","event_type":"observed_relationship","label":"An ion gradient contributes to intestinal amino-acid movement.","description":"Ex vivo pig jejunal L-threonine flux was sodium dependent in assays using 50 micromolar and 5 millimolar amino-acid concentrations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"57e6f495-f432-5a4b-bfef-35af3d9dd423","slug":"pig-jejunal-threonine-flux","display_name":"Pig jejunal mucosal-to-serosal threonine flux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Jejunum from male castrated Pietrain × Danbred pigs receiving different methionine supplements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tissue flux integrates several transporters; it does not identify one exclusive threonine carrier.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An ion gradient contributes to intestinal amino-acid movement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39408384/ · DOI 10.3390/nu16193418","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"075e1435-21e4-5a7e-b12c-faf756ea7cdd","evidence_kind":"source_excerpt","locator":"Lines 42-48","start_line":42,"end_line":48,"excerpt":"## l-threonine-pig-sodium-uptake\nAn ion gradient contributes to intestinal amino-acid movement.\nEx vivo pig jejunal L-threonine flux was sodium dependent in assays using 50 micromolar and 5 millimolar amino-acid concentrations.\nModel: Jejunum from male castrated Pietrain × Danbred pigs receiving different methionine supplements.\nLimitations: Tissue flux integrates several transporters; it does not identify one exclusive threonine carrier.\nEvidence access: Primary abstract\nTransport of Neutral Amino Acids in the Jejunum of Pigs with Special Consideration of L-Methionine. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39408384/ · DOI 10.3390/nu16193418","model_system":"Jejunum from male castrated Pietrain × Danbred pigs receiving different methionine supplements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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