{"id":"72dc68ab-4005-58a3-ab98-1535515e04d1","stable_key":"a9dd23c6-978a-5755-8bd8-f29bd1fe0cda:b3-pre-nmnat2-nmn","predicate":"catalyzes-production-of","statement":"Purified recombinant human NMNAT2 has adenylyltransferase activity toward NMN, supporting NAD+ formation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b1110ad0-9ecf-5636-ba53-6c1418d73933","mechanism_event_label":"The same human isozyme processes either the amidated or deamidated mononucleotide.","subject":{"id":"11ea02c9-58ec-54d8-948f-f89eff2c2bd9","slug":"nmnat2","display_name":"Human nicotinamide mononucleotide adenylyltransferase 2 / NMNAT2","entity_type_key":"protein"},"object":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b1110ad0-9ecf-5636-ba53-6c1418d73933","stable_key":"a9dd23c6-978a-5755-8bd8-f29bd1fe0cda:b3-pre-nmnat2-nmn-event","event_type":"biochemical_relationship","label":"The same human isozyme processes either the amidated or deamidated mononucleotide.","description":"Purified recombinant human NMNAT2 has adenylyltransferase activity toward NMN, supporting NAD+ formation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"11ea02c9-58ec-54d8-948f-f89eff2c2bd9","slug":"nmnat2","display_name":"Human nicotinamide mononucleotide adenylyltransferase 2 / NMNAT2","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d1c59139-db61-5fa0-a25d-40c3e625152e","slug":"nmn","display_name":"NMN","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"adenylyl donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/niacin-precursors-sources/nmnat2002.abstract.txt\", \"locator\": \"Indexed primary abstract\", \"start_char\": 0, \"end_char\": 826, \"file_sha256\": \"5064cc6a7b31875eeaea8def5544c3cce75a971f73aa74f332d89e06c3c54477\", \"text_sha256\": \"5064cc6a7b31875eeaea8def5544c3cce75a971f73aa74f332d89e06c3c54477\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cloned, expressed and purified recombinant human NMNAT2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Biochemical or structural assay; no dietary intervention","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme evidence does not establish dietary intake requirements or clinical outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Niacin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"niacin","display_name":"Niacin (vitamin B3)","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The same human isozyme processes either the amidated or deamidated mononucleotide.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b3-pre-nmnat2002] Identification of a novel human nicotinamide mononucleotide adenylyltransferase. (2002). https://pubmed.ncbi.nlm.nih.gov/12359228/ DOI: 10.1016/s0006-291x(02)02285-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6ddde0ea-c596-5f06-b9b5-eb302204fc4f","evidence_kind":"source_excerpt","locator":"Lines 302-313","start_line":302,"end_line":313,"excerpt":"### b3-pre-nmnat2-nmn\nPurified recombinant human NMNAT2 has adenylyltransferase activity toward NMN, supporting NAD+ formation.\nCondition category: normal\nnutrient_topic: Niacin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The same human isozyme processes either the amidated or deamidated mononucleotide.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Cloned, expressed and purified recombinant human NMNAT2\nlimitations: Purified-enzyme evidence does not establish dietary intake requirements or clinical outcomes.\nexposure: Biochemical or structural assay; no dietary intervention\nevidence_span: {\"source_cache\": \"artifacts/niacin-precursors-sources/nmnat2002.abstract.txt\", \"locator\": \"Indexed primary abstract\", \"start_char\": 0, \"end_char\": 826, \"file_sha256\": \"5064cc6a7b31875eeaea8def5544c3cce75a971f73aa74f332d89e06c3c54477\", \"text_sha256\": \"5064cc6a7b31875eeaea8def5544c3cce75a971f73aa74f332d89e06c3c54477\"}\n[b3-pre-nmnat2002] Identification of a novel human nicotinamide mononucleotide adenylyltransferase. 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