{"id":"72a6075f-9a34-5677-9177-3bbbcb37886c","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-diabetic-stem-mobilisation","predicate":"increases","statement":"Diabetic patients showed a more than twofold elevation in circulating stem cells after treatments, and the post-treatment CD34-positive population contained two- to threefold higher levels of hypoxia inducible factors 1, 2 and 3 and of thioredoxin-1.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e0107f3e-d3b6-5f12-9bf2-3a102cfad87e","mechanism_event_label":"The cells released carry more of the low-oxygen sensing proteins and more thioredoxin inside them.","subject":{"id":"581fa746-cb84-571b-87a0-4d461263f660","slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"},"object":{"id":"c984de6e-0478-527e-ab17-73e78a0ccd95","slug":"circulating-cd34-cells","display_name":"Circulating CD34-positive stem and progenitor cells","entity_type_key":"cell_type"},"evidence_count":1,"mechanism_event":{"id":"e0107f3e-d3b6-5f12-9bf2-3a102cfad87e","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-diabetic-stem-mobilisation-event","event_type":"observed_intervention","label":"The cells released carry more of the low-oxygen sensing proteins and more thioredoxin inside them.","description":"Diabetic patients showed a more than twofold elevation in circulating stem cells after treatments, and the post-treatment CD34-positive population contained two- to threefold higher levels of hypoxia inducible factors 1, 2 and 3 and of thioredoxin-1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22b3e241-94b0-55fd-a233-c63af92520d8","slug":"hif1a","display_name":"HIF-1 alpha","entity_type_key":"protein"},"role":"enriched_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ef19a1dc-9098-5e4e-99e1-2e1cd0daf283","slug":"thioredoxin","display_name":"Thioredoxin / TXN","entity_type_key":"protein"},"role":"enriched_protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"581fa746-cb84-571b-87a0-4d461263f660","slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c984de6e-0478-527e-ab17-73e78a0ccd95","slug":"circulating-cd34-cells","display_name":"Circulating CD34-positive stem and progenitor cells","entity_type_key":"cell_type"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/hbot-research/21362081.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a830ae6900d884b73f4e5eac335263db6f9d981a0f95aa45ad3e266c44800e0b\", \"start_char\": 0, \"end_char\": 1077, \"text_sha256\": \"a830ae6900d884b73f4e5eac335263db6f9d981a0f95aa45ad3e266c44800e0b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Diabetic patients undergoing hyperbaric oxygen for refractory neuropathic ulcers, with wound margin biopsies","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Clinical hyperbaric oxygen therapy courses for refractory lower-extremity ulcers","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human observational measurements within a treatment course, without a randomised comparison. 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Protein content is measured in the mobilised cells, not in healed tissue.\nexposure: Clinical hyperbaric oxygen therapy courses for refractory lower-extremity ulcers\nevidence_span: {\"source_cache\": \"artifacts/hbot-research/21362081.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a830ae6900d884b73f4e5eac335263db6f9d981a0f95aa45ad3e266c44800e0b\", \"start_char\": 0, \"end_char\": 1077, \"text_sha256\": \"a830ae6900d884b73f4e5eac335263db6f9d981a0f95aa45ad3e266c44800e0b\"}\n[hbot-p21362081] Vasculogenic stem cell mobilization and wound recruitment in diabetic patients: increased cell number and intracellular regulatory protein content associated with hyperbaric oxygen therapy. 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