{"id":"7264ce21-2f5c-5e2d-a7e4-fa55bb5f75bc","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:i-met-dio3-secis-expression","predicate":"supports","statement":"The 3-prime-untranslated-region SECIS element was required for expression of cloned human placental DIO3, which incorporated 75Se in transfected cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2fcbfa85-3134-5d51-80bd-d6c4e705c6fe","mechanism_event_label":"The hormone-inactivating enzyme also needs the cellular system that incorporates selenium into proteins.","subject":{"id":"fb35ceae-ce02-51e2-b15d-f6bb4dde263b","slug":"human-dio3-secis","display_name":"Human DIO3 3-prime-UTR SECIS element","entity_type_key":"rna"},"object":{"id":"066966e9-8e21-5e5d-9082-12e81bc4b558","slug":"dio3","display_name":"DIO3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2fcbfa85-3134-5d51-80bd-d6c4e705c6fe","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:i-met-dio3-secis-expression-event","event_type":"biochemical_relationship","label":"The hormone-inactivating enzyme also needs the cellular system that incorporates selenium into proteins.","description":"The 3-prime-untranslated-region SECIS element was required for expression of cloned human placental DIO3, which incorporated 75Se in transfected cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fb35ceae-ce02-51e2-b15d-f6bb4dde263b","slug":"human-dio3-secis","display_name":"Human DIO3 3-prime-UTR SECIS element","entity_type_key":"rna"},"role":"translation_element","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"066966e9-8e21-5e5d-9082-12e81bc4b558","slug":"dio3","display_name":"DIO3","entity_type_key":"protein"},"role":"selenoprotein_product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"42620d13-f86a-5e08-acea-7bd24787cbe4","slug":"protein-incorporated-selenocysteine","display_name":"Protein-incorporated selenocysteine residue","entity_type_key":"chemical_species"},"role":"incorporated_residue","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dc89d7e2-8f89-577b-838f-42bf5d8ec7c8","slug":"selenium","display_name":"Selenium","entity_type_key":"nutrient_element"},"role":"element_context","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Selenium incorporation supports an enzyme that inactivates iodine-containing hormones.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_document\": \"artifacts/iodine-metabolism-sources/7593630.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1405}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human placental DIO3 cDNA and heterologous expression, selenium labeling and SECIS manipulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cloned 2.1-kb DIO3 cDNA; 75Se labeling and SECIS-dependent expression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression machinery experiment; not evidence for selenium dosing in pregnancy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human placental protein in transfected cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The hormone-inactivating enzyme also needs the cellular system that incorporates selenium into proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[i-met-7593630] Type 3 lodothyronine deiodinase: cloning, in vitro expression, and functional analysis of the placental selenoenzyme. (1995). https://pubmed.ncbi.nlm.nih.gov/7593630/ DOI: 10.1172/jci118299","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"DIO3 expression system","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e702108e-aaa2-545c-92ba-fc7bcafa9a5a","evidence_kind":"source_excerpt","locator":"Lines 1129-1142","start_line":1129,"end_line":1142,"excerpt":"### i-met-dio3-secis-expression\nThe 3-prime-untranslated-region SECIS element was required for expression of cloned human placental DIO3, which incorporated 75Se in transfected cells.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The hormone-inactivating enzyme also needs the cellular system that incorporates selenium into proteins.\norganism: Human placental protein in transfected cells\ntissue_or_cell_type: DIO3 expression system\nexperimental_model: Human placental DIO3 cDNA and heterologous expression, selenium labeling and SECIS manipulation\nlimitations: Expression machinery experiment; not evidence for selenium dosing in pregnancy.\nexposure: Cloned 2.1-kb DIO3 cDNA; 75Se labeling and SECIS-dependent expression.\ncross_nutrient: Selenium incorporation supports an enzyme that inactivates iodine-containing hormones.\nevidence_locator: Primary abstract\nevidence_spans: [{\"source_document\": \"artifacts/iodine-metabolism-sources/7593630.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1405}]\n[i-met-7593630] Type 3 lodothyronine deiodinase: cloning, in vitro expression, and functional analysis of the placental selenoenzyme. 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