{"id":"725a391c-75d0-5a1e-9f46-5455188f2035","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-castor-release","predicate":"disrupts","statement":"Arginine binding disrupted the CASTOR1–GATOR2 complex; arginine-binding capacity was needed for pathway activation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d71bb5c1-16e5-5274-93b5-f49272c60425","mechanism_event_label":"Binding releases a brake on nutrient signaling.","subject":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"object":{"id":"4418d697-10f3-5e7a-a548-0d1fc6fff83a","slug":"castor1-gator2-complex","display_name":"Human CASTOR1–GATOR2 complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"d71bb5c1-16e5-5274-93b5-f49272c60425","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-castor-release-event","event_type":"observed_relationship","label":"Binding releases a brake on nutrient signaling.","description":"Arginine binding disrupted the CASTOR1–GATOR2 complex; 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tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Binding releases a brake on nutrient signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26972053/ · DOI 10.1016/j.cell.2016.02.035","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b60b491d-d89a-5ab2-b7bf-771f0f155c81","evidence_kind":"source_excerpt","locator":"Lines 182-188","start_line":182,"end_line":188,"excerpt":"## arg-castor-release\nBinding releases a brake on nutrient signaling.\nArginine binding disrupted the CASTOR1–GATOR2 complex; arginine-binding capacity was needed for pathway activation.\nModel: Mammalian-cell signaling experiments and biochemical CASTOR1 binding.\nLimitations: Do not equate pathway activation with guaranteed muscle growth.\nEvidence access: Primary abstract\nThe CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26972053/ · DOI 10.1016/j.cell.2016.02.035","model_system":"Mammalian-cell signaling experiments and biochemical CASTOR1 binding.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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