{"id":"722317eb-1220-5fba-8351-01055c9aa0fe","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-nsclc-gsh","predicate":"reduces_in_nsclc","statement":"DIM lowered cellular GSH in the lung-cancer experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7f8282be-1e97-56ec-97ae-7e1c025414b1","mechanism_event_label":"Glutathione availability changed in these cells, not as a demonstrated whole-body nutrient deficiency.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"5e8dd2eb-2ad7-519b-a0a1-0ff9ffa705da","slug":"cellular-glutathione-pool","display_name":"Cellular reduced-glutathione pool","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7f8282be-1e97-56ec-97ae-7e1c025414b1","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-nsclc-gsh-event","event_type":"biochemical_relationship","label":"Glutathione availability changed in these cells, not as a demonstrated whole-body nutrient deficiency.","description":"DIM lowered cellular GSH in the lung-cancer experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5e8dd2eb-2ad7-519b-a0a1-0ff9ffa705da","slug":"cellular-glutathione-pool","display_name":"Cellular reduced-glutathione pool","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/40100489.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"99bc986298e026c4bd40745004936bab65af37a931084f75634b89505f5b95d0\", \"start_char\": 0, \"end_char\": 1722, \"text_sha256\": \"99bc986298e026c4bd40745004936bab65af37a931084f75634b89505f5b95d0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell perturbation and xenograft experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIM; NRF2 overexpression and pharmacological rescue controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preclinical cancer context. Reduced defense here differs from Nrf2 induction in fibroblasts; neither result proves a universal antioxidant or prooxidant effect in people.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human non-small-cell lung-cancer cells and mouse xenografts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Glutathione availability changed in these cells, not as a demonstrated whole-body nutrient deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p40100489] 3,3'-diindolylmethane induces ferroptosis and inhibits proliferation in non-small-cell lung cancer through the AHR/NRF2/GPX4 axis. 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Reduced defense here differs from Nrf2 induction in fibroblasts; neither result proves a universal antioxidant or prooxidant effect in people.\nexposure: DIM; NRF2 overexpression and pharmacological rescue controls\nevidence_span: {\"source_cache\": \"artifacts/dim-research/40100489.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"99bc986298e026c4bd40745004936bab65af37a931084f75634b89505f5b95d0\", \"start_char\": 0, \"end_char\": 1722, \"text_sha256\": \"99bc986298e026c4bd40745004936bab65af37a931084f75634b89505f5b95d0\"}\n[dim-p40100489] 3,3'-diindolylmethane induces ferroptosis and inhibits proliferation in non-small-cell lung cancer through the AHR/NRF2/GPX4 axis. 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