{"id":"71c855e0-1f1f-5d72-9d26-c62733a5f1b5","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-ampa-editing","predicate":"changes","statement":"Unedited calcium-permeable AMPA assemblies had substantially larger unitary conductances than fully edited GluR2 channels in the recombinant assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4ba1e7e4-6118-5cac-b0bf-e717c76aecba","mechanism_event_label":"The receptor sequence changes how much current an excitatory signal produces.","subject":{"id":"bcba2af1-b12c-5251-9cd8-07bd8635e6e7","slug":"ampa-glur2-qr-editing-state","display_name":"GluR2 Q/R-site editing state in AMPA assays","entity_type_key":"cellular_process"},"object":{"id":"dbd1e2a5-ceec-574f-bfec-183be7c39d0c","slug":"ampa-glur2-glur4-assay-complex","display_name":"Recombinant GluR2/GluR4 AMPA receptor assay assemblies","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"4ba1e7e4-6118-5cac-b0bf-e717c76aecba","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-ampa-editing-event","event_type":"observed_relationship","label":"The receptor sequence changes how much current an excitatory signal produces.","description":"Unedited calcium-permeable AMPA assemblies had substantially larger unitary conductances than fully edited GluR2 channels in the recombinant assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bcba2af1-b12c-5251-9cd8-07bd8635e6e7","slug":"ampa-glur2-qr-editing-state","display_name":"GluR2 Q/R-site editing state in AMPA assays","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dbd1e2a5-ceec-574f-bfec-183be7c39d0c","slug":"ampa-glur2-glur4-assay-complex","display_name":"Recombinant GluR2/GluR4 AMPA receptor assay assemblies","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant GluR2/GluR4 assemblies expressed in HEK293 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Receptor construct species is not resolved in the retrieved abstract; a human host cell does not establish a human receptor sequence. Dietary calcium or amino-acid effects were not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The receptor sequence changes how much current an excitatory signal produces.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Single-channel properties of recombinant AMPA receptors depend on RNA editing, splice variation, and subunit composition. · 1997 · https://pubmed.ncbi.nlm.nih.gov/8987736/ · DOI 10.1523/JNEUROSCI.17-01-00058.1997","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"025b0bc9-8da1-56bd-b020-a5930f20b4b1","evidence_kind":"source_excerpt","locator":"Lines 242-248","start_line":242,"end_line":248,"excerpt":"## glutamate-ampa-editing\nThe receptor sequence changes how much current an excitatory signal produces.\nUnedited calcium-permeable AMPA assemblies had substantially larger unitary conductances than fully edited GluR2 channels in the recombinant assays.\nModel: Recombinant GluR2/GluR4 assemblies expressed in HEK293 cells.\nLimitations: Receptor construct species is not resolved in the retrieved abstract; a human host cell does not establish a human receptor sequence. Dietary calcium or amino-acid effects were not tested.\nEvidence access: Primary abstract\nSingle-channel properties of recombinant AMPA receptors depend on RNA editing, splice variation, and subunit composition. · 1997 · https://pubmed.ncbi.nlm.nih.gov/8987736/ · DOI 10.1523/JNEUROSCI.17-01-00058.1997","model_system":"Recombinant GluR2/GluR4 assemblies expressed in HEK293 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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