{"id":"71a56c10-ef0b-5adf-8465-1d9ab1bc8f25","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-fldc-loss","predicate":"disruption_prevents_formation","statement":"Disrupting fldC in C. sporogenes abolished indolepropionate accumulation in culture and altered aromatic-amino-acid metabolism.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1f80df2d-0978-506a-a1f9-2cf77d81be8b","mechanism_event_label":"A missing microbial enzyme stopped production despite available tryptophan.","subject":{"id":"b3e7b70f-69ce-591b-96fa-389bae9a7ed9","slug":"c-sporogenes-fldc","display_name":"Clostridium sporogenes FldC","entity_type_key":"protein"},"object":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"1f80df2d-0978-506a-a1f9-2cf77d81be8b","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-fldc-loss-event","event_type":"observed_relationship","label":"A missing microbial enzyme stopped production despite available tryptophan.","description":"Disrupting fldC in C. sporogenes abolished indolepropionate accumulation in culture and altered aromatic-amino-acid metabolism.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3e7b70f-69ce-591b-96fa-389bae9a7ed9","slug":"c-sporogenes-fldc","display_name":"Clostridium sporogenes FldC","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text, Figures 1–3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"C. sporogenes ClosTron mutant and LC–MS/MS.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The mutation affects phenylalanine and tyrosine products as well; effects cannot all be assigned to indolepropionate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A missing microbial enzyme stopped production despite available tryptophan.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29168502/ · DOI 10.1038/nature24661","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"534e890f-8944-5195-8f6e-8cdbfa8be4f6","evidence_kind":"source_excerpt","locator":"Lines 490-496","start_line":490,"end_line":496,"excerpt":"## tryptophan-fldc-loss\nA missing microbial enzyme stopped production despite available tryptophan.\nDisrupting fldC in C. sporogenes abolished indolepropionate accumulation in culture and altered aromatic-amino-acid metabolism.\nModel: C. sporogenes ClosTron mutant and LC–MS/MS.\nLimitations: The mutation affects phenylalanine and tyrosine products as well; effects cannot all be assigned to indolepropionate.\nEvidence access: Primary full text, Figures 1–3\nA gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29168502/ · DOI 10.1038/nature24661","model_system":"C. sporogenes ClosTron mutant and LC–MS/MS.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}