{"id":"7125135a-2b7e-538a-9883-2fb196c80fc7","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-bio-ppcdc-fmn","predicate":"binds_cofactor","statement":"Purified recombinant human PPCDC showed flavin-like absorbance maxima at 382 and 458 nm and approximately equimolar FMN association.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ff06bbff-4152-5c2d-a2d2-b717181fecb8","mechanism_event_label":"The CoA pathway contains a direct biochemical connection to the B2 cofactor FMN.","subject":{"id":"611d89d7-e432-5755-93c9-cef47c6b7276","slug":"ppcdc","display_name":"Phosphopantothenoylcysteine decarboxylase / PPCDC","entity_type_key":"protein"},"object":{"id":"d3eacf85-1a35-546d-bd30-161474dd9691","slug":"fmn","display_name":"Flavin mononucleotide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ff06bbff-4152-5c2d-a2d2-b717181fecb8","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-bio-ppcdc-fmn-event","event_type":"biochemical_relationship","label":"The CoA pathway contains a direct biochemical connection to the B2 cofactor FMN.","description":"Purified recombinant human PPCDC showed flavin-like absorbance maxima at 382 and 458 nm and approximately equimolar FMN association.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c6cf659d-23a5-5c8c-8ef8-79f9d5c19599","slug":"phosphopantothenoylcysteine","display_name":"4′-Phosphopantothenoyl-L-cysteine","entity_type_key":"small_molecule"},"role":"enzyme_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"role":"enzyme_product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"611d89d7-e432-5755-93c9-cef47c6b7276","slug":"ppcdc","display_name":"Phosphopantothenoylcysteine decarboxylase / PPCDC","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d3eacf85-1a35-546d-bd30-161474dd9691","slug":"fmn","display_name":"Flavin mononucleotide","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human PPCS, PPCDC and COASY expressed in Escherichia coli; coupled enzyme assays and HPLC reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified human recombinant protein spectroscopy and cofactor characterization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Direct enzyme-cofactor evidence; the study did not test dietary B2 deficiency, oral riboflavin rescue, or a universal FMN threshold. FMN origin from B2 is shared nutrient context, not a newly demonstrated dietary effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The CoA pathway contains a direct biochemical connection to the B2 cofactor FMN.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-bio-daugherty2002] Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. (2002). https://pubmed.ncbi.nlm.nih.gov/11923312/ DOI: 10.1074/jbc.m201708200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"241d7ea8-b6ef-54bd-be41-adff9ab64571","evidence_kind":"source_excerpt","locator":"Lines 561-572","start_line":561,"end_line":572,"excerpt":"### b5-bio-ppcdc-fmn\nPurified recombinant human PPCDC showed flavin-like absorbance maxima at 382 and 458 nm and approximately equimolar FMN association.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The CoA pathway contains a direct biochemical connection to the B2 cofactor FMN.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Recombinant human PPCS, PPCDC and COASY expressed in Escherichia coli; coupled enzyme assays and HPLC reconstitution\nlimitations: Direct enzyme-cofactor evidence; the study did not test dietary B2 deficiency, oral riboflavin rescue, or a universal FMN threshold. FMN origin from B2 is shared nutrient context, not a newly demonstrated dietary effect.\nexposure: Purified human recombinant protein spectroscopy and cofactor characterization.\ncross_nutrient: true\n[b5-bio-daugherty2002] Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. (2002). https://pubmed.ncbi.nlm.nih.gov/11923312/ DOI: 10.1074/jbc.m201708200","model_system":"Recombinant human PPCS, PPCDC and COASY expressed in Escherichia coli; coupled enzyme assays and HPLC reconstitution","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-bio-daugherty2002] Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. (2002). https://pubmed.ncbi.nlm.nih.gov/11923312/ DOI: 10.1074/jbc.m201708200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}