{"id":"70c8604b-8954-557a-a58c-34a3a1a6af82","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-gln-neuron","predicate":"inhibits","statement":"Theanine inhibited glutamine accumulation in cultured rat neurons.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"45f9dbc4-47a8-554d-bc87-6e67133884ed","mechanism_event_label":"Competition for a precursor can alter a neurotransmitter-related metabolic pool.","subject":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"object":{"id":"0925b77d-ac2f-54f1-a787-2a0f21181f25","slug":"neuronal-glutamine-uptake","display_name":"Neuronal glutamine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"45f9dbc4-47a8-554d-bc87-6e67133884ed","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-gln-neuron-event","event_type":"biochemical_relationship","label":"Competition for a precursor can alter a neurotransmitter-related metabolic pool.","description":"Theanine inhibited glutamine accumulation in cultured rat neurons.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"competing_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0925b77d-ac2f-54f1-a787-2a0f21181f25","slug":"neuronal-glutamine-uptake","display_name":"Neuronal glutamine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/18293419.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c94471071feadec8a04eafa7e9d53999dd9756b7f1d5dc1f351152b9b5f4b5d3\", \"start_char\": 0, \"end_char\": 1751, \"text_sha256\": \"c94471071feadec8a04eafa7e9d53999dd9756b7f1d5dc1f351152b9b5f4b5d3\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radiotracer uptake in synaptosomes and cultured neural cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Theanine 0.1-10 millimolar in uptake assays; sustained 10 millimolar for glutamate release","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High cell concentrations; 10 mM is far above the approximately 25 micromolar plasma peak in the separate human 100 mg study. Different cells from the system L screen; no claim all routes are sodium-dependent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat neurons and astroglia","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Competition for a precursor can alter a neurotransmitter-related metabolic pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p18293419] Theanine, an ingredient of green tea, inhibits [3H]glutamine transport in neurons and astroglia in rat brain. (2008). https://pubmed.ncbi.nlm.nih.gov/18293419/ DOI: 10.1002/jnr.21637","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Glutamine transport and extracellular glutamate","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2e471557-d731-530c-b675-fb6077a402a4","evidence_kind":"source_excerpt","locator":"Lines 224-235","start_line":224,"end_line":235,"excerpt":"### theanine-gln-neuron\nTheanine inhibited glutamine accumulation in cultured rat neurons.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Competition for a precursor can alter a neurotransmitter-related metabolic pool.\norganism: Rat neurons and astroglia\ntissue_or_cell_type: Glutamine transport and extracellular glutamate\nexperimental_model: Radiotracer uptake in synaptosomes and cultured neural cells\nlimitations: High cell concentrations; 10 mM is far above the approximately 25 micromolar plasma peak in the separate human 100 mg study. Different cells from the system L screen; no claim all routes are sodium-dependent.\nexposure: Theanine 0.1-10 millimolar in uptake assays; sustained 10 millimolar for glutamate release\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/18293419.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c94471071feadec8a04eafa7e9d53999dd9756b7f1d5dc1f351152b9b5f4b5d3\", \"start_char\": 0, \"end_char\": 1751, \"text_sha256\": \"c94471071feadec8a04eafa7e9d53999dd9756b7f1d5dc1f351152b9b5f4b5d3\"}\n[theanine-p18293419] Theanine, an ingredient of green tea, inhibits [3H]glutamine transport in neurons and astroglia in rat brain. (2008). https://pubmed.ncbi.nlm.nih.gov/18293419/ DOI: 10.1002/jnr.21637","model_system":"Radiotracer uptake in synaptosomes and cultured neural cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [theanine-p18293419] Theanine, an ingredient of green tea, inhibits [3H]glutamine transport in neurons and astroglia in rat brain. (2008). https://pubmed.ncbi.nlm.nih.gov/18293419/ DOI: 10.1002/jnr.21637","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2bf3fc8-fc9a-5d50-937a-559a76f8be7d","stable_key":"import-8f12ba85-1a92-5337-a9c0-9cee9b3be90d","title":"L-Theanine: metabolism, neural signaling, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c78f2a1961da546cd8d3ae9131a16613aed0d1032904ca19175b0641647142c1","revision_id":"4ec64995-1e5b-5279-9102-f203cb2e29e5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}