{"id":"70a86205-64e3-5402-9a6e-3450f83d21bf","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-gut-tas2r4","predicate":"contributes_to","statement":"Inhibitor experiments in human HuTu-80 cells supported TAS2R4 involvement in rebaudioside-A-evoked GLP-1 release.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3894820d-bc43-5cc7-8e96-473c1ec2c658","mechanism_event_label":"A bitter receptor participates in a gut-hormone response.","subject":{"id":"68bb2641-602b-5462-bbb0-96cc455be293","slug":"tas2r4","display_name":"Human bitter taste receptor / TAS2R4","entity_type_key":"protein"},"object":{"id":"46731938-4c86-5195-b463-c14c70168dfc","slug":"human-hutu80-glp1-release","display_name":"Human HuTu-80 GLP-1 release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3894820d-bc43-5cc7-8e96-473c1ec2c658","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-gut-tas2r4-event","event_type":"observed_relationship","label":"A bitter receptor participates in a gut-hormone response.","description":"Inhibitor experiments in human HuTu-80 cells supported TAS2R4 involvement in rebaudioside-A-evoked GLP-1 release.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"68bb2641-602b-5462-bbb0-96cc455be293","slug":"tas2r4","display_name":"Human bitter taste receptor / TAS2R4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"46731938-4c86-5195-b463-c14c70168dfc","slug":"human-hutu80-glp1-release","display_name":"Human HuTu-80 GLP-1 release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"16a5658c-2844-5742-9d3b-8882e5414eb6","slug":"rebaudioside-a","display_name":"Rebaudioside A","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4853afc6-9eaa-547f-91d7-c4dd90082351","slug":"glp-1","display_name":"Glucagon-like peptide 1 / GLP-1","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cell-line pharmacology; sweet-signaling inhibition did not explain the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological inhibition does not establish exclusive receptor involvement or in vivo necessity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A bitter receptor participates in a gut-hormone response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Rebaudioside A from Stevia rebaudiana stimulates GLP-1 release by enteroendocrine cells via bitter taste signalling pathways. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37431625/ · DOI 10.1039/d3fo00818e","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"adb4d186-14f0-577e-8849-c922e5c78433","evidence_kind":"source_excerpt","locator":"Lines 266-272","start_line":266,"end_line":272,"excerpt":"## stevia-gut-tas2r4\nA bitter receptor participates in a gut-hormone response.\nInhibitor experiments in human HuTu-80 cells supported TAS2R4 involvement in rebaudioside-A-evoked GLP-1 release.\nModel: Human cell-line pharmacology; sweet-signaling inhibition did not explain the response.\nLimitations: Pharmacological inhibition does not establish exclusive receptor involvement or in vivo necessity.\nEvidence access: Primary abstract\nRebaudioside A from Stevia rebaudiana stimulates GLP-1 release by enteroendocrine cells via bitter taste signalling pathways. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37431625/ · DOI 10.1039/d3fo00818e","model_system":"Human cell-line pharmacology; sweet-signaling inhibition did not explain the response.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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