{"id":"7083dcd3-29dd-5d6e-a134-a0997baec4fa","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:vessel-added-gsh-null","predicate":"no_detected_change_in_recorded_experiment","statement":"Omission of added GSH did not significantly alter the tested polysulfide-induced rat aortic relaxation.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"8c400ca6-27ac-5136-8e28-87a9d056b59d","mechanism_event_label":"Omission of added GSH did not significantly alter the tested polysulfide-induced rat aortic relaxation.","subject":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"object":{"id":"73e40f92-5b52-57cb-b7b1-10ef2b60c360","slug":"rat-garlic-aortic-relaxation","display_name":"Rat aortic-ring relaxation in the garlic-polysulfide study","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8c400ca6-27ac-5136-8e28-87a9d056b59d","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:vessel-added-gsh-null-event","event_type":"experimental_observation","label":"Omission of added GSH did not significantly alter the tested polysulfide-induced rat aortic relaxation.","description":"**Alternative thiols and the vascular null.** GSH, cysteine, homocysteine, and NAC each supported garlic-associated sulfide generation in cell-free assays. In rat aortic rings, added GSH aided detectable sulfide production, but its omission did not significantly alter the tested polysulfide-induced relaxation. Membrane and intracellular thiols therefore matter alongside the added reagent. NAC is a direct participant in this chemistry, not a selective way to raise cellular GSH. Results in deoxygenated red-cell assays and oxygen-controlled rat vessels are not human oral pharmacokinetics. [Benavides 2007](https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Polysulfide exposure without added GSH","sequence_order":0,"notes":""},{"entity":{"id":"73e40f92-5b52-57cb-b7b1-10ef2b60c360","slug":"rat-garlic-aortic-relaxation","display_name":"Rat aortic-ring relaxation in the garlic-polysulfide study","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""},{"entity":{"id":"267dc359-d357-5af4-9b4a-d4cf3f7360fc","slug":"diallyl-trisulfide","display_name":"Diallyl trisulfide / DATS","entity_type_key":"small_molecule"},"role":"tested sulfur donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"one tested donor","comparator":"Same tested polysulfide with added GSH","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"diallyl-trisulfide","display_name":"Diallyl trisulfide / DATS","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"no exogenous addition","comparator":"Same tested polysulfide with added GSH","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Polysulfide exposure without added GSH\", \"comparator\": \"Same tested polysulfide with added GSH\", \"endpoint\": \"Omission of added GSH did not significantly alter the tested polysulfide-induced rat aortic relaxation.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"glutathione\", \"state\": \"no exogenous addition\"}, {\"entity_slug\": \"diallyl-trisulfide\", \"state\": \"one tested donor\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat aortic rings; Figure 3E, DATS/DPDS comparisons with and without 1 mM added GSH.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Omitting added GSH does not remove endogenous vascular thiols. Null is relaxation, not sulfide generation; no clinical glutathione gate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Omission of added GSH did not significantly alter the tested polysulfide-induced rat aortic relaxation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Hydrogen sulfide mediates the vasoactivity of garlic. | 2007 | DOI 10.1073/pnas.0705710104 | PMID 17951430 | https://pubmed.ncbi.nlm.nih.gov/17951430/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/ | https://doi.org/10.1073/pnas.0705710104","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 81-81; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9279bcba-1b67-5c97-b20a-1a06ec06eb44","evidence_kind":"source_excerpt","locator":"Lines 81-81","start_line":81,"end_line":81,"excerpt":"**Alternative thiols and the vascular null.** GSH, cysteine, homocysteine, and NAC each supported garlic-associated sulfide generation in cell-free assays. In rat aortic rings, added GSH aided detectable sulfide production, but its omission did not significantly alter the tested polysulfide-induced relaxation. Membrane and intracellular thiols therefore matter alongside the added reagent. NAC is a direct participant in this chemistry, not a selective way to raise cellular GSH. Results in deoxygenated red-cell assays and oxygen-controlled rat vessels are not human oral pharmacokinetics. [Benavides 2007](https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/)","model_system":"Rat aortic rings; Figure 3E, DATS/DPDS comparisons with and without 1 mM added GSH.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"source_recorded","notes":""}}],"relations":[{"id":"291eabef-ee6f-594b-8af5-39aea906cf51","relation_type":"limits_translation_of","notes":"Purified chemical GSH reaction does not imply exogenous GSH is required for vascular relaxation.","source_claim_id":"7083dcd3-29dd-5d6e-a134-a0997baec4fa","source_claim_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:vessel-added-gsh-null","source_statement":"Omission of added GSH did not significantly alter the tested polysulfide-induced rat aortic relaxation.","target_claim_id":"3627c9c0-87f6-53c4-a300-8690a5a9336c","target_claim_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:dats-gsh","target_statement":"DATS reacted rapidly with GSH to release sulfide through the reported exchange and perthiol-reduction chemistry."}],"conflicts":[],"corrections":[],"research":null}