{"id":"702db102-a830-511b-b564-61fc3c2c87f0","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-zebrafish-bone-combination","predicate":"with_vitamin_d3_increases","statement":"Boron plus vitamin D3 increased opercular bone growth more than vitamin D3 alone in zebrafish larvae.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"85bfbc58-70dd-50a8-8e60-ed3b529e8b5b","mechanism_event_label":"The combination outperformed vitamin D alone in developing fish.","subject":{"id":"5144a867-de17-599d-baec-68500e3d40bd","slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"},"object":{"id":"83cae1a2-5e3b-592e-9749-d8a95e5a14bd","slug":"zebrafish-opercular-bone-growth","display_name":"Zebrafish opercular bone growth","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"85bfbc58-70dd-50a8-8e60-ed3b529e8b5b","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-zebrafish-bone-combination-event","event_type":"observed_intervention","label":"The combination outperformed vitamin D alone in developing fish.","description":"Boron plus vitamin D3 increased opercular bone growth more than vitamin D3 alone in zebrafish larvae.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b162e431-b442-5400-9335-ec91f7875a4e","slug":"cholecalciferol","display_name":"Cholecalciferol","entity_type_key":"small_molecule"},"role":"co_exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5144a867-de17-599d-baec-68500e3d40bd","slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"83cae1a2-5e3b-592e-9749-d8a95e5a14bd","slug":"zebrafish-opercular-bone-growth","display_name":"Zebrafish opercular bone growth","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/35571926.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"cf22409e40b52d79a9f1738a942771e626afdb39fce0e8bc16cbf877cdf7ea30\", \"start_char\": 0, \"end_char\": 2097, \"text_sha256\": \"cf22409e40b52d79a9f1738a942771e626afdb39fce0e8bc16cbf877cdf7ea30\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Larval zebrafish morphology, transcriptomics and fluorescent osteoblast reporters","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Waterborne boron 10 or 100 ng/mL, vitamin D3 10 pg/mL and combinations; developmental time points","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Fish water exposure cannot be translated directly into human supplement intake. Combination superiority alone is not proof of a formal statistical interaction; pathway enrichment is not proof of direct molecular activation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Danio rerio","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The combination outperformed vitamin D alone in developing fish.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p35571926] Zebrafish as a Model to Unveil the Pro-Osteogenic Effects of Boron-Vitamin D3 Synergism. (2022). https://pubmed.ncbi.nlm.nih.gov/35571926/ DOI: 10.3389/fnut.2022.868805","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Opercular bone and osteoblast development","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7954d813-baaf-530c-9b55-193ea0a92ad8","evidence_kind":"source_excerpt","locator":"Lines 885-896","start_line":885,"end_line":896,"excerpt":"### boron-zebrafish-bone-combination\nBoron plus vitamin D3 increased opercular bone growth more than vitamin D3 alone in zebrafish larvae.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The combination outperformed vitamin D alone in developing fish.\norganism: Danio rerio\ntissue_or_cell_type: Opercular bone and osteoblast development\nexperimental_model: Larval zebrafish morphology, transcriptomics and fluorescent osteoblast reporters\nlimitations: Fish water exposure cannot be translated directly into human supplement intake. Combination superiority alone is not proof of a formal statistical interaction; pathway enrichment is not proof of direct molecular activation.\nexposure: Waterborne boron 10 or 100 ng/mL, vitamin D3 10 pg/mL and combinations; developmental time points\nevidence_span: {\"source_cache\": \"artifacts/boron-research/35571926.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"cf22409e40b52d79a9f1738a942771e626afdb39fce0e8bc16cbf877cdf7ea30\", \"start_char\": 0, \"end_char\": 2097, \"text_sha256\": \"cf22409e40b52d79a9f1738a942771e626afdb39fce0e8bc16cbf877cdf7ea30\"}\n[boron-p35571926] Zebrafish as a Model to Unveil the Pro-Osteogenic Effects of Boron-Vitamin D3 Synergism. (2022). https://pubmed.ncbi.nlm.nih.gov/35571926/ DOI: 10.3389/fnut.2022.868805","model_system":"Larval zebrafish morphology, transcriptomics and fluorescent osteoblast reporters","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p35571926] Zebrafish as a Model to Unveil the Pro-Osteogenic Effects of Boron-Vitamin D3 Synergism. (2022). https://pubmed.ncbi.nlm.nih.gov/35571926/ DOI: 10.3389/fnut.2022.868805","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2d96c547-aeec-5d8b-9d31-1288da209f89","stable_key":"import-7edf94bb-95c8-5234-9161-9eb338bb9b36","title":"Boron: chemistry, nutrient interactions, low-intake studies and mechanistic uncertainties (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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