{"id":"700e12d2-b2af-54e7-9bfb-f490852f36ec","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-knockdown-glucose","predicate":"silencing_reduces","statement":"Silencing CRAT in human myotubes reduced glucose uptake by 13% while increasing oleate oxidation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f1d5dfbf-8013-567e-936f-03284af4fa66","mechanism_event_label":"More fat oxidation did not mean better glucose handling in this experiment.","subject":{"id":"507b3d2c-db75-51d1-823a-0677e0c1a0e8","slug":"human-crat","display_name":"Human carnitine acetyltransferase / CRAT","entity_type_key":"protein"},"object":{"id":"0647ee42-3746-5709-bedf-a013f3a880e3","slug":"human-myotube-glucose-uptake","display_name":"Human primary myotube glucose uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f1d5dfbf-8013-567e-936f-03284af4fa66","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-knockdown-glucose-event","event_type":"observed_relationship","label":"More fat oxidation did not mean better glucose handling in this experiment.","description":"Silencing CRAT in human myotubes reduced glucose uptake by 13% while increasing oleate oxidation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"507b3d2c-db75-51d1-823a-0677e0c1a0e8","slug":"human-crat","display_name":"Human carnitine acetyltransferase / CRAT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0647ee42-3746-5709-bedf-a013f3a880e3","slug":"human-myotube-glucose-uptake","display_name":"Human primary myotube glucose uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full-text Figure 6","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human myotube loss-of-function experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No universal reciprocal rule for all tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"More fat oxidation did not mean better glucose handling in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Muscle-specific deletion of carnitine acetyltransferase compromises glucose tolerance and metabolic flexibility. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560225/ · DOI 10.1016/j.cmet.2012.04.005","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fbb4fe5b-c0c7-57aa-b42d-4a3c004594c0","evidence_kind":"source_excerpt","locator":"Lines 178-184","start_line":178,"end_line":184,"excerpt":"## l-carnitine-crat-knockdown-glucose\nMore fat oxidation did not mean better glucose handling in this experiment.\nSilencing CRAT in human myotubes reduced glucose uptake by 13% while increasing oleate oxidation.\nModel: Primary human myotube loss-of-function experiment.\nLimitations: No universal reciprocal rule for all tissues.\nEvidence access: Primary full-text Figure 6\nMuscle-specific deletion of carnitine acetyltransferase compromises glucose tolerance and metabolic flexibility. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560225/ · DOI 10.1016/j.cmet.2012.04.005","model_system":"Primary human myotube loss-of-function experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"6fcd86d9d02babb48b2ce658d506afe84580ad4b242f93f11f742d934fa5b361","revision_id":"398e3b52-45bc-52e3-891e-656c19ca674f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}