{"id":"6f95b27d-02ac-578a-9344-fd15a5793101","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-dmgdh-thf","predicate":"binds","statement":"Rat DMGDH structures located THF in a domain separated from the FAD-containing active center and connected by an internal channel.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"76fef6d0-5080-5eb6-b582-2995724b42c4","mechanism_event_label":"Folate and a riboflavin-derived cofactor occupy distinct sites in the same enzyme.","subject":{"id":"818f4c70-a0ce-5ce6-8a4b-804eb9796448","slug":"rat-dmgdh","display_name":"Rat dimethylglycine dehydrogenase / Dmgdh","entity_type_key":"protein"},"object":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"76fef6d0-5080-5eb6-b582-2995724b42c4","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-dmgdh-thf-event","event_type":"biochemical_relationship","label":"Folate and a riboflavin-derived cofactor occupy distinct sites in the same enzyme.","description":"Rat DMGDH structures located THF in a domain separated from the FAD-containing active center and connected by an internal channel.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"enzyme_flavin","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"818f4c70-a0ce-5ce6-8a4b-804eb9796448","slug":"rat-dmgdh","display_name":"Rat dimethylglycine dehydrogenase / Dmgdh","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/24858690.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e00bbb8f1c2c805e9d7261c286bace5758abd277bc95b3e0a830bd9cdb82a34f\", \"start_char\": 0, \"end_char\": 1506, \"text_sha256\": \"e00bbb8f1c2c805e9d7261c286bace5758abd277bc95b3e0a830bd9cdb82a34f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat DMGDH crystallography and enzyme characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dimethylglycine turnover and THF-bound structures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species and assay context retained. The proposed intramolecular transfer route is not represented as a directly observed moving intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat recombinant enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Folate and a riboflavin-derived cofactor occupy distinct sites in the same enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p24858690] Folate in demethylation: the crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate. (2014). https://pubmed.ncbi.nlm.nih.gov/24858690/ DOI: 10.1016/j.bbrc.2014.05.064","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial-enzyme preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a10d89c9-e9da-5c89-8d0e-8504a5afdf2d","evidence_kind":"source_excerpt","locator":"Lines 672-683","start_line":672,"end_line":683,"excerpt":"### choline-dmgdh-thf\nRat DMGDH structures located THF in a domain separated from the FAD-containing active center and connected by an internal channel.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Folate and a riboflavin-derived cofactor occupy distinct sites in the same enzyme.\norganism: Rat recombinant enzyme\ntissue_or_cell_type: Mitochondrial-enzyme preparation\nexperimental_model: Rat DMGDH crystallography and enzyme characterization\nlimitations: Species and assay context retained. The proposed intramolecular transfer route is not represented as a directly observed moving intermediate.\nexposure: Dimethylglycine turnover and THF-bound structures\nevidence_span: {\"source_cache\": \"artifacts/choline-research/24858690.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e00bbb8f1c2c805e9d7261c286bace5758abd277bc95b3e0a830bd9cdb82a34f\", \"start_char\": 0, \"end_char\": 1506, \"text_sha256\": \"e00bbb8f1c2c805e9d7261c286bace5758abd277bc95b3e0a830bd9cdb82a34f\"}\n[choline-p24858690] Folate in demethylation: the crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate. (2014). https://pubmed.ncbi.nlm.nih.gov/24858690/ DOI: 10.1016/j.bbrc.2014.05.064","model_system":"Rat DMGDH crystallography and enzyme characterization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [choline-p24858690] Folate in demethylation: the crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate. (2014). https://pubmed.ncbi.nlm.nih.gov/24858690/ DOI: 10.1016/j.bbrc.2014.05.064","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0ba2f05e-5cf5-5bd7-ae5d-0ab4945fcb7b","stable_key":"import-27e0c1cf-7726-5164-8b15-27a631584cd0","title":"Choline: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c7afe3d579eba0df6d2a4c26699c653462749e03188348f900c07748cc7282a8","revision_id":"0fa7b48f-2a3c-5adc-b13e-48727dd6f815","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}