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(2022). https://pubmed.ncbi.nlm.nih.gov/34813684/ DOI: 10.1111/febs.16291","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"MK-4 production and protein carboxylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"8f779b5c-6b87-5a7c-bd90-e6ddbfde9043","evidence_kind":"source_excerpt","locator":"Lines 253-264","start_line":253,"end_line":264,"excerpt":"### k2-ubiad1-g186r\nUBIAD1 G186R significantly impaired both MK-4 biosynthesis and vitamin K-dependent reporter carboxylation.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin K2 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A different variant impaired both supply and use in the same assay.\norganism: Human HEK293 reporter cells\ntissue_or_cell_type: MK-4 production and protein carboxylation\nexperimental_model: CRISPR reporter cells with UBIAD1 variants\nlimitations: Cell-specific residual activity differs between variants; a UBIAD1 mutation is not automatically global vitamin K failure.\nexposure: UBIAD1 N102S, G186R and other variants\nevidence_span: {\"source_cache\": \"artifacts/k2-research/34813684.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f533947abd5f26101d1bf1e2548d37b98d16bb72c470dcdfaf2b2f2625c5e206\", \"start_char\": 0, \"end_char\": 1777, \"text_sha256\": \"f533947abd5f26101d1bf1e2548d37b98d16bb72c470dcdfaf2b2f2625c5e206\"}\n[k2-p34813684] Naturally occurring UBIAD1 mutations differentially affect menaquinone biosynthesis and vitamin K-dependent carboxylation. 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