{"id":"6f7c7326-32dc-59e7-b91d-3ddbafb6fa36","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-deprivation-early-ammonium","predicate":"deprivation-increases","statement":"Potassium-deficient feeding increased urinary ammonium excretion before detectable hypokalemia in the studied rats.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"dc426fe7-dfbb-5ec1-a33d-49cd5c94dc19","mechanism_event_label":"The kidney changed nitrogen and acid excretion before blood potassium fell.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"c11a5503-bb50-5cc3-ae50-4c0660d52cfb","slug":"urinary-ammonium-excretion","display_name":"Urinary ammonium excretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dc426fe7-dfbb-5ec1-a33d-49cd5c94dc19","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-deprivation-early-ammonium-event","event_type":"biochemical_relationship","label":"The kidney changed nitrogen and acid excretion before blood potassium fell.","description":"Potassium-deficient feeding increased urinary ammonium excretion before detectable hypokalemia in the studied rats.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c11a5503-bb50-5cc3-ae50-4c0660d52cfb","slug":"urinary-ammonium-excretion","display_name":"Urinary ammonium excretion","entity_type_key":"cellular_process"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e923077b-9786-5ab9-b23b-638777ac6a85","slug":"ammonium-ion","display_name":"Ammonium ion","entity_type_key":"ion"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"edf44f6b-7b23-5b97-a86b-c940e5812b06","slug":"bicarbonate-ion","display_name":"Bicarbonate ion","entity_type_key":"ion"},"role":"associated-pathway-product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Potassium deprivation changes glutamine nitrogen metabolism and sodium-coupled glutamine transport; these experiments did not manipulate dietary protein adequacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"Potassium-deficient feeding increased urinary ammonium excretion before detectable hypokalemia in the studied rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Dietary deprivation precedes detectable hypokalemia here; protein expression does not independently establish transport flux or quantify net bicarbonate generation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The kidney changed nitrogen and acid excretion before blood potassium fell.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hossain-2011-k-ammoniagenesis] Cellular and molecular basis of increased ammoniagenesis in potassium deprivation (2011). https://journals.physiology.org/doi/full/10.1152/ajprenal.00010.2011 DOI: 10.1152/ajprenal.00010.2011","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"renal proximal tubule and whole-kidney excretion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f3a96320-c46a-5053-93d7-15626762ef0e","evidence_kind":"source_excerpt","locator":"Lines 958-970","start_line":958,"end_line":970,"excerpt":"### k-deprivation-early-ammonium\nPotassium-deficient feeding increased urinary ammonium excretion before detectable hypokalemia in the studied rats.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The kidney changed nitrogen and acid excretion before blood potassium fell.\norganism: Rattus norvegicus\ntissue_or_cell_type: renal proximal tubule and whole-kidney excretion\nexperimental_model: Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.\nlimitations: Dietary deprivation precedes detectable hypokalemia here; protein expression does not independently establish transport flux or quantify net bicarbonate generation.\ncross_nutrient: Potassium deprivation changes glutamine nitrogen metabolism and sodium-coupled glutamine transport; these experiments did not manipulate dietary protein adequacy.\nexperimental-exposure: Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.\nendpoint: Potassium-deficient feeding increased urinary ammonium excretion before detectable hypokalemia in the studied rats.\n[hossain-2011-k-ammoniagenesis] Cellular and molecular basis of increased ammoniagenesis in potassium deprivation (2011). https://journals.physiology.org/doi/full/10.1152/ajprenal.00010.2011 DOI: 10.1152/ajprenal.00010.2011","model_system":"Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hossain-2011-k-ammoniagenesis] Cellular and molecular basis of increased ammoniagenesis in potassium deprivation (2011). https://journals.physiology.org/doi/full/10.1152/ajprenal.00010.2011 DOI: 10.1152/ajprenal.00010.2011","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}