{"id":"6f5a30d8-211d-5bb5-9114-c3036da194c7","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-keap1-cetsa","predicate":"shows_cellular_engagement_of","statement":"A cellular thermal-shift assay supported engagement of KEAP1 by SAC in human colonic epithelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"db4faf12-a7b4-55f3-8d52-6158991f5bdc","mechanism_event_label":"A target-engagement assay gives a more direct molecular lead.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"c5b67b38-fe93-5e8f-91af-d77d3f590d98","slug":"keap1","display_name":"Human KEAP1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"db4faf12-a7b4-55f3-8d52-6158991f5bdc","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-keap1-cetsa-event","event_type":"observed_relationship","label":"A target-engagement assay gives a more direct molecular lead.","description":"A cellular thermal-shift assay supported engagement of KEAP1 by SAC in human colonic epithelial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c5b67b38-fe93-5e8f-91af-d77d3f590d98","slug":"keap1","display_name":"Human KEAP1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC with PhIP challenge and corresponding cell controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Normal human colonic mucosal epithelial cells exposed to PhIP","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Thermal stabilization does not alone identify a binding site, affinity, covalent adduct or purified-protein mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A target-engagement assay gives a more direct molecular lead.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[35753083] S-Allylcysteine Potently Protects against PhIP-Induced DNA Damage via Nrf2/AhR Signaling Pathway Modulation in Normal Human Colonic Mucosal Epithelial Cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35753083/ · DOI 10.1002/mnfr.202101141","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Cell culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Normal human colonic mucosal epithelial cells exposed to PhIP","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"46ef34b6-fc57-517d-b7eb-ae0fd99db78b","evidence_kind":"source_excerpt","locator":"Lines 149-156","start_line":149,"end_line":156,"excerpt":"## s-allylcysteine-keap1-cetsa\nA target-engagement assay gives a more direct molecular lead.\nA cellular thermal-shift assay supported engagement of KEAP1 by SAC in human colonic epithelial cells.\nModel: Normal human colonic mucosal epithelial cells exposed to PhIP\nLimitations: Thermal stabilization does not alone identify a binding site, affinity, covalent adduct or purified-protein mechanism.\nEvidence access: Primary abstract\n[35753083] S-Allylcysteine Potently Protects against PhIP-Induced DNA Damage via Nrf2/AhR Signaling Pathway Modulation in Normal Human Colonic Mucosal Epithelial Cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35753083/ · DOI 10.1002/mnfr.202101141\nStructured context: {\"organism\": \"Homo sapiens\", \"tissue_or_cell_type\": \"Normal human colonic mucosal epithelial cells exposed to PhIP\", \"dose\": \"Not specified in accessed abstract\", \"duration\": \"Not specified in accessed abstract\", \"route\": \"Cell culture\", \"experimental_comparison\": \"SAC with PhIP challenge and corresponding cell controls\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Normal human colonic mucosal epithelial cells exposed to PhIP","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"91d7aeac82e42ce559d13dd8a2e0165d37760f7f555dd01c80e0604fa6e71ea2","revision_id":"545e497c-e5e3-543c-b26b-b297cbc7bbec","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}